EFFECTS OF TGF-β1 ON CELLULAR PROLIFERATION ANDEXPRESSION OF TYPE Ⅲ COLLAGEN BY CULTURED RATRENAL INTERSTITIAL FIBROBLASTS

被引：0

作者：

姚建

王伟铭

石蓉

楼鼎

董德长

机构：

来源：

Journal of Shanghai Second Medical University | 1999年 / 01期

关键词：

TGF-; β1; type Ⅲ collagen mRNA; renal interstitial fibroblast; cellularproliferation;

D O I：

暂无

中图分类号：

R329.2 [人体细胞学];

学科分类号：

100101 ;

摘要：

Objective To investigate the effects of TGF- β1 on proliferation and type N collagen mRNAexpression of rat renal interstitial libroblasts in vitro for elucidating the role of fibroblasts in renal interstitialfibrosis. Methods The cells were cultured in media containing various concentrations of TGF- β1. Theproliferation and the type Ⅲ collagen mRNA expression of the cells were assayed with MTT method andRT- PCR respectively. Results TGF- β1, has a stimulating proliferation effect with dose- dependence and aincreasing expression of type Ⅲ collagen mRNA with both dose- and time- dependence to the renal fibroblasts. Conclusion It is suggested that TGF- β, is involved in proliferation of renal fibroblasts and their type Ⅲcollagen mRNA expression, and may play an important role in renal interstitial fibrosis.

引用

页码：39 / 41 +50

页数：4

共 8 条

[1]

Rodemann HP,Binder A,Burger A,et al.The underlyingcellular mechanism of fibrosis. Kidney International . 1996

[2]

Strutz F,Neilson EG.The role of lymphocytes in theprogression of interstitial disease. Kidney International . 1994

[3]

Eddy AA.Expression of genes that promote renal interstitial fibrosis in rats with proteinuria. Kidney International . 1996

[4]

Clayton A,Steadman R,Williams JD.Cells isolated fromthe human conical interstitum resemble myofibroblasts andbind neutrophils in an ICAM -1- dependent manner. Journal of the American Society of Nephrology . 1997

[5]

Lonnemann G,Shapiro L,Engler - Blum G,et al.Cytokinsin human renal interstitial fibrosis I.Interleukin - 1 is aparacrine growth factor for cultured fibrosis - derivedkidney fibroblasts. Kidney International . 1995

[6]

Bohle A,Muller GA,Wehrmann M,et al.Pathogenesis ofchronic renal failure in the primary glomerulopathies, renalvasculopathies, and chronic interstitial nephritides. Kidney International . 1996

[7]

D’Angelo M,Chen JM,Ward G,et al.Regulation of type Ⅲcollagen metabolism by transforming growth factor β inembryonic cells. The Journal of Cell Biology . 1991

[8]

Terada Y,Tomita K,Nonoguchi H,et al.Expression ofendothin- 3 mRNA along rat nephron segments using polymerase chain reaction.Kidney Int, 1993; 44:12737. Eddy AA. Molecular insights into renal interstitial fibrosis. Journal of the American Society of Nephrology . 1996

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