国外栽培豌豆遗传多样性分析及核心种质构建

被引：31

作者：

宗绪晓 ^{[1
]}

关建平 ^{[2
]}

王述民 ^{[2
]}

刘庆昌 ^{[1
]}

Robert R Redden ^{[3
]}

Rebecca Ford ^{[4
]}

机构：

[1] 中国农业大学农学与生物技术学院

[2] 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程

[3] Australian Temperate Field Crops Collection,Grains Innovation Park,The Department of Primary Industries,Private Bag , Horsham,Victoria,Australia

[4] BioMarka,Faculty of Land and Food Resources,The University of Melbourne,Victoria,Australia

来源：

作物学报 | 2008年 / 09期

关键词：

豌豆栽培种(Pisum sativum L.); SSR; 遗传多样性; 核心种质; 国外资源;

D O I：

暂无

中图分类号：

S643.3 [豌豆];

学科分类号：

090202 ;

摘要：

从111对备选SSR引物中筛选出能扩增出清晰稳定单一带的多态性引物21对及其最佳退火温度,并优化了豌豆SSR标记实验体系。利用上述引物,对来自于67个国家的731份豌豆栽培种质(Pisum sativum L.)进行遗传多样性分析与核心种质构建。共扩增出109条多态性带,每对引物平均扩增出5.19个等位变异。SSR等位变异在各大洲间分布不均匀,有效等位变异数、Shannon's信息指数(I)洲际间差异明显。各大洲资源群间遗传多样性差异显著,其中亚洲最高(I=1.1753),欧洲其次(I=1.1387),俄罗斯联邦(I=1.0285)、美洲(I=1.0196)、非洲(I=0.9254)、大洋洲(I=0.8608)依次降低。利用Popgene1.32软件,依豌豆栽培资源洲际间Nei78遗传距离可聚类成2个组群和4个亚组群;基于Structure 2.2软件分析,国外栽培豌豆资源实际由3大类群组成,并与Popgene 1.32聚类结果呼应得较好。上述两种分析方法均表明,国外栽培豌豆类群的遗传多样性与其地理分布相关。设计并实践了一套基于Structure分析的科学可靠、逻辑性强的核心种质构建标准化方案,并依此构建了一套以6.57%的资源(48份)涵盖总体84.4%等位变异的国外栽培豌豆核心种质。

引用

页码：1518 / 1528

页数：11

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