Bioinformatics Analysis of Genes and Pathways of CD11b~+/Ly6Cintermediate Macrophages after Renal Ischemia-Reperfusion Injury

被引:0
作者
孙冬 [1 ]
万辛 [1 ]
潘斌斌 [1 ]
孙晴 [2 ]
嵇小兵 [1 ]
张峰 [1 ]
张浩 [1 ]
曹长春 [1 ]
机构
[1] Department of Nephrology,Nanjing First Hospital,Nanjing Medical University
[2] Department of Nephrology,The Affiliated Sir Run Run Hospital,Nanjing Medical University
基金
中国国家自然科学基金;
关键词
renal ischemia-reperfusion injury; macrophage; differentially expressed genes; series test of cluster; functional enrichment analysis; protein-protein interaction;
D O I
暂无
中图分类号
R692 [肾疾病];
学科分类号
1002 ; 100210 ;
摘要
Renal ischemia-reperfusion injury(IRI) is a major cause of acute kidney injury(AKI),which could induce the poor prognosis.The purpose of this study was to characterize the molecular mechanism of the functional changes of CD11 b+/Ly6 Cintermediate macrophages after renal IRI.The gene expression profiles of CD11 b+/Ly6 Cintermediate macrophages of the sham surgery mice,and the mice 4 h,24 h and 9 days after renal IRI were downloaded from the Gene Expression Omnibus database.Analysis of m RNA expression profiles was conducted to identify differentially expressed genes(DEGs),biological processes and pathways by the series test of cluster.Protein-protein interaction network was constructed and analysed to discover the key genes.A total of 6738 DEGs were identified and assigned to 20 model profiles.DEGs in profile 13 were one of the predominant expression profiles,which are involved in immune cell chemotaxis and proliferation.Signet analysis showed that Atp5 a1,Atp5 o,Cox4 i,Cdc42,Rac2 and Nhp2 were the key genes involved in oxidation-reduction,apoptosis,migration,M1-M2 differentiation,and proliferation of macrophages.RPS18 may be an appreciate reference gene as it was stable in macrophages.The identified DEGs and their enriched pathways investigate factors that may participate in the functional changes of CD11 b+/Ly6 Cintermediate macrophages after renal IRI.Moreover,the vital gene Nhp2 may involve the polarization of macrophages,which may be a new target to affect the process of AKI.
引用
收藏
页码:70 / 77
页数:8
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