大豆中L34基因的克隆与表达分析

被引：1

作者：

李淑艳 ^{[1
,2
]}

王建中 ^{[1
]}

机构：

[1] 北京林业大学生物学院

[2] 孝感学院生命科学技术学院

来源：

生物技术 | 2009年 / 19卷 / 04期

关键词：

大豆; L34基因; 克隆; 表达;

D O I：

10.16519/j.cnki.1004-311x.2009.04.026

中图分类号：

S565.1 [大豆]; Q943.2 [植物基因工程];

学科分类号：

0901 ; 071007 ; 090102 ;

摘要：

目的:采用分子手段试图分离与大豆种子低温吸胀相关的基因。方法:利用cDNA-AFLP(cDNA-amplified fragmentlength polymorphism)和RACE技术从大豆种子胚轴中克隆到一个编码132 kD的全长核糖体蛋白基因,命名为SOL34。结果:序列分析表明,SOL34和苜蓿(gi|113205273|)、茄科植物(gi|48057670|)及拟南芥(gi|2500376|)中L34蛋白基因的同源性分别为95%、95%和90%。半定量RT-PCR结果表明:大豆种子在4℃下吸胀24h内,胚轴中的SOL34被诱导表达,其中当种子低温吸胀6h,SOL34表达量升高非常明显,18h的表达量最大,24h表达量和18h相似;SOL34在大豆不同部位的表达不同,4叶期的大豆幼苗经过低温处理后,根尖中SOL34的表达比非处理材料增强约5倍,同时比胚轴中高约2倍;但是在叶片中,SOL34表达量并没有受到温度的影响,表达量也很弱,说明SOL34在叶片中是组成型表达。结论:结果分析表明,SOL34可能和大豆根的代谢有关。

引用

页码：3 / 6

页数：4

共 24 条

[1]

Variation in traits associated with chilling tolerance during emergence in cowpea germplasm[J] . Abdelbagi M. Ismail,Anthony E. Hall. &nbspField Crops Research . 2002 (2)

[2]

Chilling injury in germinating seeds: basic mechanisms and agricultural implications[J] . Seema Bedi,Amarjit Singh Basra. &nbspSeed Science Research . 1993 (4)

[3]

Ribosomal protein gene regu-lation:what about plants?. Kerri B Mclntosh,Peta C Bonham-Smith. Canadian Journal of Botany . 2006

[4]

Mutation in Saccharomyces cerevisiae conferring streptomycin and cold sensitivity by affecting ribosome formation and function. Bayliss,F.T.,Ingraham,J.L. Journal of Bacteriology . 1974

[5]

Sequencing and characterization of the Xenopus laevis ribosomal protein L34 cDNA[J] . Maria Carmela Vaccaro. &nbspGene . 2003

[6]

GMCHI , cloned from soybean [ Glycine max （L.） Meer.], enhances survival in transgenic Arabidopsis under abiotic stress[J] . Libao Cheng,Shutao Huan,Yaodi Sheng,Xuejun Hua,Qingyan Shu,Songquan Song,Xinming Jing. &nbspPlant Cell Reports . 2009 (1)

[7]

Sequencing and characterization of the Xenopus laevis ribosomal protein L34 cDNA. Vaccaro MC. Gene . 2003

[8]

The two ribosomal protein L23A genes are differentially transcribed in Arabidopsis thaliana. KB McIntosh,PC Bonham-Smith. Genome . 2005

[9]

Identification of genesup-regulated in response to Cd exposure in Brassicajuncea L. Lang ML,Zhang YX,Chai TY. Gene . 2005

[10]

Study on the imbibitional chilling injury andthe reparation of damaged membrane systems in soybeanseeds. Zheng G H. Scientia Sinica . 1988

← 1 2 3 →