兴奋性氨基酸受体的激活介导冷水应激诱导的tau蛋白磷酸化(英文)附视频

被引：11
作者：
武凤英
冯琼
程敏
闫洁
许玉霞
朱粹青
机构：
[1] 复旦大学上海医学院医学神经生物学国家重点实验室
来源：
生物化学与生物物理进展 | 2010年 / 05期
关键词：
冷水应激; Tau蛋白磷酸化; 兴奋性氨基酸神经递质; HPLC;
D O I：
暂无
中图分类号：
Q42 [神经生理学];
学科分类号：
071006 ;
摘要：
为探讨兴奋性神经传递系统是否参与冷水应激引起的tau蛋白磷酸化,将小鼠于4℃冷水应激5min.采用免疫印迹和免疫组织化学方法分析应激后脑内c-fos和磷酸化tau蛋白的表达情况;运用HPLC检测冷水应激后小鼠脑内兴奋性或抑制性神经递质的变化;同时分析兴奋性氨基酸受体和L-型钙通道拮抗剂预处理后冷水应激小鼠脑内磷酸化tau蛋白的水平.冷水应激后1h,海马内磷酸化tau蛋白的水平显著升高,同时伴c-fos的染色增加.HPLC检测显示,兴奋性和抑制性神经递质呈现急剧上升而后又下降的趋势.冷水应激后15min,天冬氨酸和甘氨酸水平显著升高,1h后天冬氨酸、谷氨酸、牛磺酸和γ-氨基丁酸显著下降.NMDA受体拮抗剂MK-801(5mg/kg)和AMPA受体拮抗剂DNQX(0.5,5mg/kg)可显著抑制冷水应激引起的磷酸化tau蛋白水平的升高,代谢性谷氨酸受体拮抗剂MAP-4不影响tau蛋白的磷酸化,另外,L-型钙通道阻断剂尼莫地平可抑制冷水应激引起的磷酸化tau蛋白水平的升高.这些结果表明,冷水应激可影响兴奋性神经传递系统,通过离子型兴奋性氨基酸受体和异常神经激活来调节tau蛋白的磷酸化.兴奋性神经传递系统的激活在冷水应激诱导的海马tau蛋白的磷酸化中发挥作用.
引用
收藏
页码：510 / 516
页数：7
相关论文
共 12 条
[1]   Dynamic changes of phosphorylated tau in mouse hippocampus after cold water stress [J].
Feng, Q ;
Cheng, B ;
Yang, R ;
Sun, FY ;
Zhu, CQ .
NEUROSCIENCE LETTERS, 2005, 388 (01) :13-16
[2]   Proneness to psychological distress and risk of Alzheimer disease in a biracial community [J].
Wilson, RS ;
Barnes, LL ;
Bennett, DA ;
Li, Y ;
Bienias, JL ;
de Leon, CFM ;
Evans, DA .
NEUROLOGY, 2005, 64 (02) :380-382
[3]   N-methyl-D-aspartate receptors enhance mechanical responses and voltage-dependent Ca2+ channels in rat dorsal root ganglia neurons through protein kinase C [J].
Chaban, VV ;
Li, J ;
Ennes, HS ;
Nie, J ;
Mayer, EA ;
Mcroberts, JA .
NEUROSCIENCE, 2004, 128 (02) :347-357
[4]  
Overexpression of tau leads to the stimulation of neurite outgrowth, the activation of caspase 3 activity, and accumulation and phosphorylation of tau in neuroblastoma cells on cAMP treatment[J] . Chihiro Yoshizaki,Mariko Tsukane,Takashi Yamauchi.Neuroscience Research . 2004 (4)
[5]  
Stress-induced hyperphosphorylation of tau in the mouse brain[J] . Yasuhiro Okawa,Koichi Ishiguro,Shinobu C Fujita.FEBS Letters . 2003 (1)
[6]   Okadaic acid mediates tau phosphorylation via sustained activation of the L-voltage- sensitive calcium channel [J].
Ekinci, FJ ;
Ortiz, D ;
Shea, TB .
MOLECULAR BRAIN RESEARCH, 2003, 117 (02) :145-151
[7]   MK 801 attenuates c-Fos and c-Jun expression after in vitro ischemia in rat neuronal cell cultures but not in PC 12 cells [J].
Gerlach, R ;
Beck, M ;
Zeitschel, U ;
Seifert, V .
NEUROLOGICAL RESEARCH, 2002, 24 (07) :725-729
[8]  
Release patterns of excitatory and inhibitory amino acids within the hypothalamic supraoptic nucleus in response to direct nitric oxide administration during forced swimming in rats[J] . Mario Engelmann,Gerald Wolf,Thomas F.W. Horn.Neuroscience Letters . 2002 (3)
[9]  
Starvation induces tau hyperphosphorylation in mouse brain: implications for Alzheimer’s disease[J] . Masahiro Yanagisawa,Emmanuel Planel,Koichi Ishiguro,Shinobu C Fujita.FEBS Letters . 1999 (3)
[10]   Tau protein pathology in neurodegenerative diseases [J].
Spillantini, MG ;
Goedert, M .
TRENDS IN NEUROSCIENCES, 1998, 21 (10) :428-433
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