基于mtDNA和rDNA基因序列的中国按蚊属塞蚊亚属种类的系统发育研究(双翅目:蚊科)

被引:11
作者
吴静
马雅军
马颖
机构
[1] 第二军医大学病原生物学教研室
关键词
按蚊属; 塞蚊亚属; 分子系统学; mtDNA; rDNA; 系统发育;
D O I
10.16380/j.kcxb.2010.09.006
中图分类号
Q969 [昆虫分类学];
学科分类号
07 ; 0710 ; 09 ;
摘要
【目的】应用mtDNA和rDNA基因特征重建中国按蚊属塞蚊亚属已知种类的系统发育关系,以阐明亚属内各蚊种的亲缘关系。【方法】对采自中国的按蚊属塞蚊亚属Anopheles(Cellia)20种蚊的mtDNA-COⅡ和rDNA-28S-D3序列进行测定和分析,以按蚊属按蚊亚属Anopheles(Anopheles)的中华按蚊An.(An.)sinensis和赫坎按蚊An.(An.)hyrcanus为外群,采用COⅡ和D3单基因,以及"COⅡ+D3"联合数据组以邻接法(NJ)、最大简约法(MP)、最大似然法(ML)和贝叶斯法(BI)等重建这些种类的系统发育树。【结果】mtDNA-COⅡ和rDNA-28S-D3序列的长度范围分别为685bp和375~410bp,在塞蚊亚属蚊种间的遗传距离分别为0.015~0.117和0.003~0.111。各系统树显示外群被合理分开,除在COⅡ树中新塞蚊系为并系外,各系均聚为单系群,新迈蚊系和迈蚊系亲缘关系最近。联合数据组构建的系统合意树显示中国塞蚊亚属各蚊种形成4支,除伪威氏按蚊与多斑按蚊种团未聚为单系群外,其他各种团和复合体成员种均分别聚在一起,各分支的置信值均大于50%。【结论】本研究获得的分子系统发育树清楚地显示了中国按蚊属塞蚊亚属各种类及系之间的系统发育关系,对其分类和防治研究具有参考价值。
引用
收藏
页码:1030 / 1038
页数:9
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