补阳还五汤对大鼠局灶性脑缺血损伤血脑屏障的影响

被引：28
|
作者：
饶晓
汤轶波
潘彦舒
刘希伟
刘洋
池名
马英姿
牟天龙
机构：
[1] 北京中医药大学基础医学院
来源：
中国中医药信息杂志 | 2014年 / 21卷 / 06期
基金：
国家自然科学基金重点项目;
关键词：
补阳还五汤; 局灶性脑缺血; 血脑屏障; 基质金属蛋白酶-2; 基质金属蛋白酶-9; 血管内皮生长因子; 血管性血友病因子; 大鼠;
D O I：
暂无
中图分类号：
R285.5 [中药实验药理];
学科分类号：
摘要：
目的观察补阳还五汤对大鼠局灶性脑缺血损伤血脑屏障的保护作用,阐明该方抗脑缺血的机理。方法采用线栓法复制大鼠局灶性脑缺血模型。将SD大鼠随机分为假手术组、模型组和补阳还五汤组,造模后24 h给药,补阳还五汤组给予补阳还五汤灌胃,假手术组、模型组给予等量生理盐水灌胃。造模后7 d,评价各组大鼠神经功能并处死取材,四氮唑红染色观察大鼠脑组织梗死体积,HE染色观察大鼠脑微血管的一般形态,ELISA检测大鼠脑组织内基质金属蛋白酶(MMP)-9、MMP-2、血管内皮生长因子(VEGF)蛋白水平及血清中血管性血友病因子(vWF)的表达。结果与模型组比较,补阳还五汤组能显著改善神经功能行为评分,减少脑梗死体积,降低MMP-9、MMP-2、VEGF、vWF水平。病理观察结果显示,补阳还五汤组脑组织病理变化较模型组减轻。结论补阳还五汤对大鼠局灶性脑缺血具有保护作用,其作用可能与保护血脑屏障,抑制MMP-9、MMP-2、VEGF、vWF的表达有关。
引用
收藏
页码：49 / 52
页数：4
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