Retrograde Labeling of Adult Rat Retinal Ganglion Cells with the Flurogold

<正> Purpose: To study the densities and distribution of retinal ganglion cells (RGC) in adultrat retinae with flurogold(FG) labeling retogradely.Methods: FG was injected to the superior colliculi(SC) and dorsal lateral geniculatenuclei (dLGN) in adult rats and the retinae were examined by fluorescence microscopyat various periods of time.Results: FG-labelled RGC were observed in the retina as early as 3 days after applicationof FG. The labelled cells gradually increased in density, reached 95% of the maximalnumber on days 7 and the maximal number on days 30. The density of labelled cellswas higher in the posterior pole than in the peripheral area. The fluorescence intensity inlabelled cells maintained up to 60 days.Conclusion: The FG retrograde labeling method is reliable and effective for quantity ofRGC. Eye Science 2000; 16: 29 - 33.