Expression and clinical significance of calcitonin receptor stimulating peptide-3(CRSP-3) in placenta tissue of preeclampsia patients

Objective To study clinical significance and the difference of calcitonin receptor stimulating peptide-3(CRSP-3) expression in placenta tissue between normal pregnancies and patients with preeclampsia.Methods CRSP-3 expression position in placenta tissues of 50 cases with preeclampsia(preeclampsia group: 25 cases of mild preeclampsia and 25 cases of severe preeclampsia) and 30 cases of normal late pregnancy(control group) was detected using immunohistochemical methods. CRSP-3 m RNA and protein expression in placenta tissues was detected using Real-time PCR and Western blotting method.Results Syncytiotrophoblast and cytotrophoblast cells expressed CRSP-3 in placenta tissue of preeclampsia and normal pregnancy patients. The expression levels of CRSP-3 mR NA and protein in placenta tissue of preeclampsia patients were higher than those in control group(P<0.05). The expression levels of CRSP-3 mR NA and protein in placenta tissue of severe preeclampsia patients were higher than those in control group(P<0.01) significantly. The expression level of CRSP-3 mR NA and protein in placenta tissue of severe preeclampsia group was slightly higher than that in mild preeclampsia group, but there were no statistical differences between the two groups(P>0.05).Conclusion The up-regulation of CRSP-3 in placenta of preeclamptic patients may be associated with the etiology of preeclampsia, but may have no relation with progress and degree of preeclampsia.