Comparative Several Extraction Methods of Genomic DNA from Tripterygium wilfordii Hook. f. Leaves

[Objective] Comparison of the extraction methods of genomic DNA from the leaves of Tripterygium wilfordii Hook. f. [Method] With leaves of Tripterygium wilfordii Hook. f. as tested material, the genomic DNA was extracted using five different methods: CTAB method, modified CTAB method, SDS method, high salt and low pH method and ROSE method. The purity of the DNA was examined by agarose gel electrophoresis, OD260/OD280 and RAPD amplification, and the yield of the DNA was estimated by OD260. [Result] Modified CTAB method was the optimum one for DNA extraction. The ratio of OD260 and OD280 of the genomic DNA extracted by modified CTAB method was 1.869-1.903, and showed good performance in RAPD analysis. [Conclusion] The modified CTAB method is an optimum method for the extraction of genomic DNA from Tripterygium wilfordii Hook. f.