Difference in Expression of Bcl-2 and Bcl-xl Genes in Cisplatin-sensitive and Cisplatin-resistant Human in Ovarian Cancer Cell Lines

被引：0

作者：

于利利

王泽华

机构：

[1] Tongji Medical College

[2] Huazhong University of Science and Technology Wuhan 430022

[3] Union Hospital

[4] Department of Obstetrics and Gynecology

[5] China

[6] Huazhong University of Science and Technology

来源：

华中科技大学学报(医学英德文版) | 2004年 / 02期

关键词：

ovarian cancer; apoptosis; multidrug-resistance; Bcl-2 and Bcl-xl; cisplatin;

D O I：

暂无

中图分类号：

R737.31 [卵巢肿瘤];

学科分类号：

100214 ;

摘要：

To investigate the expression of Bcl-2 and Bcl-xl gene in sensitive (A2780) and drug-resistance (AD6) human ovarian cancer cell lines and explore the molecular mechanism of multidrug resistance, A2780 and AD6 were detected by using DNA gel electrophoresis, flow cytometry and RT-PCR. Our results showed that (1)"DNA ladder " was observed in A2780 and AD6 after cisplatin treatment; (2) after 3.0, 6.0, 9.9 μg/ml of cisplatin treatment, a significant difference was noted in the rate of apoptosis between in A2780 and AD6 (P<0.05); (3) Bcl-2 and Bcl-xl genes were overexpressed in AD6. After cisplatin treatment, the expression of Bcl-2 and Bcl-xl genes was down-regulated in A2780 and AD6. It is concluded that cisplatin could induce the apoptosis of ovarian cancer cells, and the over-expression of Bcl-2 and Bcl-xl genes may contribute to apoptotic inhibition and the development of multidrug-resistance of human ovarian cancer.

引用

页码：151 / 153

页数：3

共 9 条

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