Difference in Expression of Bcl-2 and Bcl-xl Genes in Cisplatin-sensitive and Cisplatin-resistant Human in Ovarian Cancer Cell Lines

被引：0

作者：

于利利

王泽华

机构：

[1] Department of Obstetrics and Gynecology

[2] Union Hospital

[3] Tongji Medical College

[4] Huazhong University of Science and Technology

[5] Huazhong University of Science and Technology Wuhan

[6] China

[7] Wuhan

来源：

华中科技大学学报(医学英德文版) | 2004年 / 02期

关键词：

ovarian cancer; apoptosis; multidrug-resistance; Bcl-2 and Bcl-xl; cisplatin;

D O I：

暂无

中图分类号：

R737.31 [卵巢肿瘤];

学科分类号：

100214 ;

摘要：

To investigate the expression of Bcl-2 and Bcl-xl gene in sensitive (A2780) and drug-resistance (AD6) human ovarian cancer cell lines and explore the molecular mechanism of multidrug resistance, A2780 and AD6 were detected by using DNA gel electrophoresis, flow cytometry and RT-PCR. Our results showed that (1)"DNA ladder " was observed in A2780 and AD6 after cisplatin treatment; (2) after 3.0, 6.0, 9.9 μg/ml of cisplatin treatment, a significant difference was noted in the rate of apoptosis between in A2780 and AD6 (P<0.05); (3) Bcl-2 and Bcl-xl genes were overexpressed in AD6. After cisplatin treatment, the expression of Bcl-2 and Bcl-xl genes was down-regulated in A2780 and AD6. It is concluded that cisplatin could induce the apoptosis of ovarian cancer cells, and the over-expression of Bcl-2 and Bcl-xl genes may contribute to apoptotic inhibition and the development of multidrug-resistance of human ovarian cancer.

引用

页码：151 / 153

页数：3

共 9 条

[1]

Guang,S Z,Tu,C Q,Zhang,G Y.Indomethacin induces apoptosis and inhibits proliferation in chronic myloid leukmia cells. Leukemia Research . 2000

[2]

Miyashita,T,Reed,J C.Bcl-2 oncoprotein blocks chemotherapy-induced apoptosis in a human leukemia cell line. Blood . 1993

[3]

Rajnee A,Mihir T,Wendi L et al.Fas-activated apoptosis and apoptosis mediators in human trabecular meshwork cells. Experimental Eye Research . 1999

[4]

Alessandro,Q,Laura,P,Valeria,S.Quantilation of bcl-2 oncogene in cultured lymphomal/leukemia cell lines and in primary leukemia B-cells by a highly sensitive RT-PCR method. Hematological Oncology . 1995

[5]

Mesner P W Jr,Budihardjo I I,Kaufmann S H.Chemotherapy-induced apoptosis. Advances in Pharmacology . 1997

[6]

Oltvai,Z N,Milliman,C L,Korsmeyer,S J.Bcl-2 heterodimerizesin vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell . 1993

[7]

Kuhl J S,Krajewski S,Duran G E et al.Spontaneous overexpression of the long form of the bcl-x protein in a highly resistant p388 leukemia. British Journal of Cancer . 1997

[8]

Cure H,Bay J O,Plagne R et al.High-dose chemotherapy in advanced epithelial ovarian cancer. Bulletin du Cancer . 2001

[9]

Meyn,R E,Stephens,L C,Hunter,M R.Kinetics of cisplatin-induced apoptosis in murine mammary and ovarian adenocarcinomas. International Journal of Cancer . 1995

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