FoxP3、T-bet及GATA-3在重症肌无力发病中的作用

被引:0
|
作者
谢娟
罗六一
徐文华
任明山
机构
[1] 上海交通大学医学院附属新华医院崇明分院
关键词
重症肌无力; 转录因子; 翼状螺旋转录因子P3; GATA3转录因子;
D O I
暂无
中图分类号
R746.1 [重症肌无力];
学科分类号
摘要
目的探讨转录因子翼状螺旋转录因子P3(FoxP3)、表达于T细胞的T盒转录因子(T-bet)和可与DNA序列A/T(GATA)A/G结合的Ⅳ型锌指蛋白3(GATA-3)在自身免疫性重症肌无力(EAMG)发病机制中的作用。方法将30只Lewis大鼠随机分为对照组13只和EAMG组17只。EAMG组采用Rα97-116肽段三次免疫方法建立EAMG模型12只。ELISA法测定两组血浆TGF-β、IFN-γ、IL-4表达水平,RT-PCR法检测外周血单个核细胞中(PBMC)FoxP3、T-bet、GATA-3 mRNA表达强度。结果与对照组比较,EAMG组T-bet mRNA、GATA-3mRNA、IFN-γ、IL-4表达水平升高(P<0.05);FoxP3 mRNA、TGF-β表达下降(P<0.05)。结论 EAMG大鼠PBMC中T-bet、GATA-3高表达、FoxP3低表达可能是EAMG细胞和体液免疫异常的重要原因之一。
引用
收藏
页码:25 / 27
页数:3
相关论文
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