Effects of Meloxicam on Vascular Endothelial Growth Factor and Angiopoietin-2 Expression in Colon Carcinoma Cell Line HT-29

To investigate the effect of meloxicam,a selected NSAIDs,on cell growth,expression of VEGF and angiopointin-2(Ang-2) protein in HT-29 cell line,cultured HT-29 cells were treated with meloxicam of various concentrations for various lengths of time.The proliferation of HT-29 was de-tected by cell counting kit-8(CCK-8),the cell cycle was determined by flow cytometer and the levels of VEGF and Ang-2 protein in supernatants were examined by enzyme linked immunosorbent assay(ELISA).The mRNA expressions of VEGF and Ang-2 in cultured HT-29 were determined by real-time quantitative reverse-transcription polymerase chain reaction.Our results showed that treat-ment of meloxicam of different concentrations and for various lengths of time had a cytotoxicic effect on the cell proliferation of HT-29 cells in a concentration-dependant and time-dependant manner.Cell cycle analysis showed that the cells were mainly blocked in G0/G1 phase.The VEGF and Ang-2 pro-tein levels in supernatants of the culture medium were decreased gradually in a concentra-tion-dependent or time-dependent fashion.The mRNA expression of cox-2,VEGF and Ang-2 showed a gradual and concentration-dependent reduction.It is concluded that meloxicam can reduce the ex-pression of VEGF and Ang-2 at the protein and mRNA level in colon carcinoma cell line.