Fut8基因通过促进VLA-4/VCAM-1表达影响B细胞发育

被引：4

作者：

焦鑫艳

马彪

王旭

刘庆平

李文哲

机构：

[1] 不详

[2] 大连大学生命科学与技术学院免疫研究所

[3] 不详

来源：

免疫学杂志 | 2012年 / 08期

关键词：

VLA-4; VCAM-1; 前B细胞; Fut8; 相互作用;

D O I：

10.13431/j.cnki.immunol.j.20120158

中图分类号：

R392.11 [免疫分子生物学（免疫化学）];

学科分类号：

摘要：

目的探讨核心岩藻糖基化修饰对VLA-4/VCAM-1表达及B细胞发育的影响。方法通过RNA干扰技术使前B细胞(70Z/3)和基质细胞(ST2)的Fut8基因沉默,免疫沉淀和Western blot检测Fut8基因沉默效率,以细胞黏附实验和克隆形成实验分别研究Fut8基因对VLA-4/VCAM-1之间亲和力和对B细胞分化发育的影响。结果 Fut8基因沉默使VLA-4/VCAM-1之间的亲和力以及前B细胞和基质细胞之间的黏附作用降低,并使前B细胞的克隆形成能力明显下降。流式细胞仪分析结果也表明,Fut8-/-祖B细胞向前B细胞分化过程受阻。结论本实验揭示了Fut8基因调节VLA-4/VCAM-1之间结合能力以及前B细胞克隆形成的机理,为B细胞分化发育研究提供理论依据。

引用

页码：683 / 686

页数：4

共 18 条

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