人CD34和CD34细胞在NOD/SCID小鼠体内的造血重建

被引:0
作者
金慧丽
蔡海波
杨施
谭文松
机构
[1] 华东理工大学生物反应器工程国家重点实验室
关键词
CD34-细胞; CD34+细胞; NOD/SCID小鼠; 造血重建能力;
D O I
10.13345/j.cjb.2008.09.008
中图分类号
Q813 [细胞工程];
学科分类号
摘要
利用非肥胖糖尿病型重症联合免疫缺陷型(NOD/SCID)小鼠模型,比较了新鲜及培养后的CD34+和CD34-细胞在体内植入及重建造血能力。从新鲜脐血及培养后的单个核细胞(MNC)中分离出CD34+和CD34-细胞,经尾静脉输注入经亚致死剂量照射的NOD/SCID小鼠体内,6周后处死存活的小鼠,取其骨髓、脾脏和外周血细胞,分别进行细胞表型分析、造血集落形成单位和人特异性基因的检测。经检测,输注CD34+细胞和混合细胞的小鼠,其体内CD45+细胞及人源各系血细胞的含量相近,两者均远远高于输注CD34-细胞的小鼠。输注培养后CD34-细胞的小鼠饲养6周后全部死亡,输注培养后CD34+细胞的小鼠存活率约为66.7%,而输注培养后混合细胞的小鼠全部存活,且在两组存活的小鼠体内均能检测到CD45+细胞及人源各系血细胞。结果表明:无论是新鲜还是培养后的CD34+细胞均具有在NOD/SCID小鼠体内植入和重建造血能力,而CD34-细胞不具有该能力,但CD34-细胞与CD34+细胞同时输注有助于提高小鼠的存活率,说明其对CD34+细胞在小鼠体内发挥植入和造血重建能力有一定的辅助作用。
引用
收藏
页码:1588 / 1594
页数:7
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