E-cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in human epithelial cells

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作者：

XU XiaoyongSHI YiZHANG PengpengZHANG FengSHEN YuyingSU Xin and ZHAO Beilei Department of Respiratory MedicineSecond Military Medical UniversityNanjing General Hospital of Nanjing Military CommandPeoples Liberation ArmyNanjingJiangsu China ^{[210002
]}

机构：

来源：

中华医学杂志（英文版） | 2012年 / 04期

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中图分类号：

R379 [病原真菌（霉菌）与放线菌];

学科分类号：

100103 ; 100705 ;

摘要：

<正>Background Aspergillus fumigatus {A.fumigatus) is a ubiquitous saprophytic fungus responsible for the majority of invasive mold infections in patients undergoing chemotherapy,organ transplantation or with persistent neutropenia.This study aimed to determine the role of E-cadherin for adhesion and endocytosis of A.fumigatus blastospores in the human epithelial cell line A549. Methods A.fumigatus blastospores were incubated with the total protein of A549 to investigate the binding of E-cadherin and blastospores followed by an affinity purification procedure.After establishing the adhesion model,the adhesion and endocytosis of A.fumigatus blastospores by A549 cells were evaluated by down-regulating E-cadherin of A549 cells using blocking antibody or small interfering RNA(siRNA). Results E-cadherin was adhered to the surface of A.fumigatus blastospore.Adhesion and endocytosis of the blastospores were reduced by blocking or down-regulating E-cadherin in A549 cells. Conclusions E-cadherin is a receptor for adhesion and endocytosis of A.fumigatus blastospores in epithelial cells. This may open a new approach to treat this fungal infection.

引用

页码：617 / 621

共 4 条

[1]

Cadherins as novel targets for anti-cancer therapy[J] . Orest W. Blaschuk,Emmanuelle Devemy. European Journal of Pharmacology . 2009 (1)

[2]

E-cadherin is a receptor for the common protein pneumococcal surface adhesin A (PsaA) of Streptococcus pneumoniae[J] . Julie M. Anderton,Gowrisankar Rajam,Sandra Romero-Steiner,Susan Summer,Andrew P. Kowalczyk,George M. Carlone,Jacquelyn S. Sampson,Edwin W. Ades. Microbial Pathogenesis . 2007 (5)

[3] Interaction of airway epithelial cells (A549) with spores and mycelium of Aspergillus fumigatus [J].

Zhang, ZH ;

Liu, RY ;

Noordhoek, JA ;

Kauffman, HF .

JOURNAL OF INFECTION, 2005, 51 (05) :375-382

[4]

Structure of Internalin, a Major Invasion Protein of Listeria monocytogenes, in Complex with Its Human Receptor E-Cadherin[J] . Wolf-Dieter Schubert,Claus Urbanke,Thilo Ziehm,Viola Beier,Matthias P. Machner,Eugen Domann,Jürgen Wehland,Trinad Chakraborty,Dirk W. Heinz. Cell . 2002 (6)

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