Effects of CXCR7-neutralizing antibody on neurogenesis in the hippocampal dentate gyrus and cognitive function in the chronic phase of cerebral ischemia

被引：0

作者：

Bing-Chao Dong ^{[1
]}

Mei-Xuan Li ^{[1
]}

Xiao-Yin Wang ^{[1
]}

Xi Cheng ^{[1
]}

Yu Wang ^{[1
]}

Ting Xiao ^{[2
]}

Jukka Jolkkonen ^{[3
]}

Chuan-Sheng Zhao ^{[1
]}

Shan-Shan Zhao ^{[1
]}

机构：

[1] Department of Neurology, The First Hospital of China Medical University

[2] Key Laboratory of Immunodermatology, Ministry of Health, Ministry of Education

[3] Department of Neurology, Institute of Clinical Medicine, University of Eastern Finland

来源：

NeuralRegenerationResearch | 2020年 / 15卷 / 06期

基金：

中国国家自然科学基金;

关键词：

cerebral ischemia; cognitive function; CXCR7; dendritic development; dentate gyrus; doublecortin; neurogenesis; neutralizing antibody; stroke; stromal cell-derived factor-1;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

1002 ;

摘要：

Stromal cell-derived factor-1 and its receptor CXCR4 are essential regulators of the neurogenesis that occurs in the adult hippocampal dentate gyrus.However,the effects of CXCR7,a new atypical receptor of stromal cell-derived factor-1,on hippocampal neurogenesis after a stroke remain largely unknown.Our study is the first to investigate the effect of a CXCR7-neutralizing antibody on neurogenesis in the dentate gyrus and the associated recovery of cognitive function of rats in the chronic stage of cerebral ischemia.The rats were randomly divided into sham,sham+anti-CXCR7,ischemia and ischemia+anti-CXCR7 groups.Endothelin-1 was injected in the ipsilateral motor cortex and striatum to induce focal cerebral ischemia.Sham group rats were injected with saline instead of endothelin-1 via intracranial injection.Both sham and ischemic rats were treated with intraventricular infusions of CXCR7-neutralizing antibodies for 6 days 1 week after surgery.Immunofluorescence staining with doublecortin,a marker for neuronal precursors,was performed to assess the neurogenesis in the dentate gyrus.We found that anti-CXCR7 antibody infusion enhanced the proliferation and dendritic development of doublecortin-labeled cells in the dentate gyrus in both ischemic and sham-operated rats.Spatial learning and memory functions were assessed by Morris water maze tests 30-32 days after ischemia.CXCR7-neutralizing antibody treatment significantly reduced the escape latency of the spatial navigation trial and increased the time spent in the target quadrant of spatial probe trial in animals that received ischemic insult,but not in sham operated rats.These results suggest that CXCR7-neutralizing antibody enhances the neurogenesis in the dentate gyrus and improves the cognitive function after cerebral ischemia in rats.All animal experimental protocols and procedures were approved by the Institutional Animal Care and Use Committee of China Medical University(CMU16089 R) on December 8,2016.

引用

页码：1079 / 1085

页数：7

共 34 条

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