普通小麦TaDep1基因克隆与特异性标记开发

被引：8
作者：
刘亚男 [1 ]
夏先春 [1 ]
何中虎 [1 ,2 ]
机构：
[1] 中国农业科学院作物科学研究所/国家小麦改良中心
[2] CIMMYT中国办事处
来源：
作物学报 | 2013年 / 39卷 / 04期
关键词：
普通小麦; 直立密穗基因; 等位变异; 基因特异性标记;
D O I：
暂无
中图分类号：
S512.1 [小麦];
学科分类号：
0901 ;
摘要：
OsDep1(dense and erectpanicle 1)控制水稻产量性状,影响穗长、直立性和着粒密度。根据水稻OsDep1基因序列,采用同源克隆技术克隆了普通小麦第5同源群染色体上的TaDep1基因,它包含5个外显子和4个内含子,与水稻OsDep1基因结构相似。TaDep1-A1、TaDep1-B1和TaDep1-D1的编码序列长度分别为918、888和900bp,编码305、295和299个氨基酸残基。在普通小麦品种中检测到5个TaDep1-A1等位变异、4个TaDep1-B1等位变异和2个TaDep1-D1等位变异。根据TaDep1-A1和TaDep1-B1位点不同等位变异间的SNP和InDel,开发了3对显性互补标记和1个共显性标记,可以准确鉴别不同等位基因。共显性标记dep19是根据TaDep1-B1第5外显子一个30bp的InDel开发的,可准确区分TaDep1-B1c与TaDep1-B1a、TaDep1-B1b和TaDep1-B1d。用这些标记对406份小麦品种进行检测,不同基因型的千粒重、株高、穗长、小穗数和穗节间均差异不显著,说明TaDep1基因与我国现有小麦
引用
收藏
页码：589 / 598
页数：10
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