Single Nucleotide Polymorphism of FSHβ Gene Associated with Reproductive Traits in Japanese Flounder (Paralichthys olivaceus)

被引：0
作者：
HE Feng WEN Haishen YU Dahui LI Jifang SHI Bao CHEN Caifang ZHANG Jiaren JIN Guoxiong CHEN Xiaoyan Shi Dan and Yang Yanping South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou PRChina Fisheries College Ocean University of China Qingdao PRChina [1 ,2 ,2 ,1 ,2 ,2 ,2 ,2 ,2 ,2 ,2 ,2 ,1 ,510300 ,2 ,266003 ]
机构：
来源：
JournalofOceanUniversityofChina | 2010年 / 9卷 / 04期
关键词：
Japanese flounder; FSHβ; SNP; reproductive trait;
D O I：
暂无
中图分类号：
S917.4 [水产动物学];
学科分类号：
0908 ;
摘要：
Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development.This study aimed to investigate molecular genetic characteristics of the FSHβ gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on reproductive traits in Japanese flounder.We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSHβ gene in 60 individuals.We identified only an SNP (T/C) in the coding region of exon3 of FSHβ.The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSHβ gene.Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) (P<0.05).Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI (P<0.05) than that of genotype CC.Therefore, FSHβ gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder.
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页码：395 / 398
页数：4
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