miR-125b-5p靶向STAT3调节JAK抑制骨髓增生异常综合征细胞增殖

被引：1

作者：

刘小敏 ^{[1
]}

杨美玲 ^{[2
]}

施俊柱 ^{[1
]}

机构：

[1] 深圳市龙华区中心医院医学检验科

[2] 深圳市龙华区人民医院急诊重症监护室

来源：

解剖科学进展 | 2022年 / 28卷 / 04期

关键词：

miR-125b-5p; 骨髓增生异常综合征; 细胞增殖; STAT3; JAK;

D O I：

10.16695/j.cnki.1006-2947.2022.04.007

中图分类号：

R551.3 [骨髓疾病];

学科分类号：

摘要：

目的探究miR-125b-5p对骨髓增生异常综合征细胞增殖的作用及可能机制。方法 qRTPCR检测骨髓增生异常综合征患者骨髓组织miR-125b-5p表达；将miR-125b-5p模拟物转染骨髓增生异常综合征SKM-1细胞，CCK-8和Edu实验检测细胞增殖；qRT-PCR检测STAT3、JAK1和JAK2 mRNA表达；Western blot检测STAT3、JAK1和JAK2蛋白表达；生物信息学预测STAT3 3'非翻译区miR-125b-5p潜在结合位点，并通过双荧光素酶报告基因实验验证。结果 miR-125b-5p在骨髓增生异常综合征患者骨髓组织中表达上调。上调miR-125b-5p抑制SKM-1细胞增殖，抑制SKM-1细胞STAT3、JAK1和JAK2表达。miR-125b-5p靶向结合STAT3。结论miR-125b-5p通过靶向抑制STAT3表达调节JAK抑制骨髓增生异常综合征细胞增殖。

引用

页码：401 / 404

页数：4

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