Molecular cloning of pheromone biosynthesis activating neuropeptide in silkworm, Bombyx mori

被引:0
作者
徐卫华
Yukihiro Stao
Okitsugu Yamashita
不详
机构
[1] Sericultural Research Institute
[2] Chinese Academy of Agricultural Sciences
[3] Zhenjiang
[4] China
[5] Laboratory of Sericultural Sciences
[6] School of Agriculture
[7] Nagoya University
[8] Nagoya -
[9] Japan
关键词
pheromone biosynthesis activating neuropeptide; molecular cloning; Bombyx mori;
D O I
暂无
中图分类号
Q785 [转化及克隆];
学科分类号
071007 ; 0836 ; 090102 ;
摘要
<正> Pheromone biosynthesis activating neuropeptide (PBAN) is a suboesophageal ganglion secretory polypeptide of insect, which activates the pheromone gland to produce sex pheromone biosynthesis in female silkworm, Bombyx mori. A Bombyx genomic library was screened by the method of plaque hybridization using the 32P-labeled BomDH cDNA as a probe. The genomic sequence encoding PBAN has been cloned and its structure is analyzed. The PBAN gene comprises two exons interspersed by a single intron 697 bp in length. Preceding the PBAN amino acid sequence is a 32-amino acid sequence containing two FXPRL amide peptides, which are α-SGNP (Ile-Ile-Phe-Thr-Pro-Lys-Leu) and β-SGNP (Ser-Val-Ala-Asn-Pro-Arg-Thr-His-Glu-Ser-Leu-Glu-Phe-Ile-Pro-Arg-Leu), which is followed by a Gly-Arg processing site. Immediately, after the PBAN amino acid sequence is a Gly-Arg processing site and a FXPRL amide peptide γ-SGNP (Thr-Met-Ser-Phe-Ser-Pro-Arg-Leu). It is suggested that besides PBAN, 7-, 8-, and 17-residue amidated peptides wer
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页码:63 / 70
页数:8
相关论文
共 2 条
[1]  
Imai,K. et al. Proc. Japan Acad . 1991
[2]  
Soroker,V. et al. Insect Biochem . 1989