乳酸菌在不同培养基中产抗氧化酶活性的研究

将10株乳酸菌分别接种到MRS肉汤、鲜牛乳、蔬菜汁培养基中进行发酵。发酵48h后用羟胺法检测超氧化物歧化酶,可见光法检测过氧化氢酶的活力。结果表明:在MRS肉汤培养基中,LA,LCP,BL,LC,LP,LCR,ST,LR,SF,LB产SOD酶和CAT酶活力分别为38.45,36.24,26.21,28.43,14.62,17.52,12.53,14.82,9.82,15.21U/mL和8.56,7.23,5.36,3.46,2.09,2.12,1.25,2.49,1.49,2.23U/mL;在鲜牛乳培养基中产SOD酶和CAT酶活力分别为50.34,47.51,43.26,39.45,19.98,14.92,21.03,13.62,17.24,17.03U/mL和9.89,8.56,6.57,4.03,2.96,2.98,2.25,3.02,2.83,3.12U/mL;在蔬菜汁培养基中产SOD酶和CAT酶活力分别为118.56,115.42,104.34,99.58,82.25,80.91,80.03,76.20,82.03,78.92U/mL和38.84,41.06,28.53,31.27,21.25,22.03,12.51,15.12,11.12,2.73U/mL。