Effects of the Cell Cycle Inhibitor Olomoucine on Inflammatory Response and Neuronal Cell Death after Spinal Cord Injury in Rats

被引：1

作者：

TIAN DaishiXIE MinjieYU ZhiyuanZHANG QiangWANG YihuiCHEN BinCHEN ChenWANG Wei Department of NeurologyTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan China ^{[430030
]}

机构：

来源：

神经损伤与功能重建 | 2007年 / 03期

关键词：

cell cycle; spinal cord injury; microglial cell; inflammatory response; neuronal apoptosis;

D O I：

暂无

中图分类号：

R614 [麻醉学];

学科分类号：

100217 ;

摘要：

Objective:The influence of olomoucine on microglial proliferation with associated inflammatory response after spinal cord injury has been determined.Methods:Microglial proliferation and neuronal apoptosis were observed by immunofluorescence.Level of the proinflammatory cytokine interleukin-1β(IL-1β)expression in the injured cord was determined by Western blot analysis.Results:the cell cycle inhibitor olomoucine,administered at 1 h post injury,significantly suppressed microglial proliferation and produced a remarkable reduction of tissue edema formation.In the olomoucine-treated group,a significant reduction of activated and/or proliferated microglial induced IL-1β expression was observed 24 h after SCI.Moreover,olomoucine evidently attenuated the number of apoptotic neurons after SCI.Conclusion:Our findings suggest that modulation of microglial proliferation with associated proinflammatory cytokine expression may be a mechanism of cell cycle inhibition-mediated neuroprotections in the CNS trauma.

引用

页码：143 / 151

页数：9

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