Total Synthesis and Expressions in E. coli of Three Redesigned Genes for the Mature Small Subunit (rbcS) of Rubisco Fron Tobacco, Wheat and Rice

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CHEN HaiBao Shanghai Institute of Organic Chemistry Academia Sinica Shanghai [200032 ]
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gene design; synthetic gene; gene expression; RubisCO; rbcS;
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<正> Three structural genes, which code for the mature small subunits (rbcS) of ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco, wheat and rice, respectively,have been redesignedwith the aid of a computer,totally synthesized by combining chemical method and enzymatic ligation,and expressed in E. coli with high yields.
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页码:309 / 313
页数:5
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