The genetic diversity analysis in the donkey myostatin gene

被引：0

作者：

LIU Donghua ^{[1
,2
]}

HAN Haoyuan ^{[1
]}

ZHANG Xin ^{[1
]}

SUN Ting ^{[1
]}

LAN Xianyong ^{[1
]}

CHEN Hong ^{[1
]}

LEI Chuzhao ^{[1
]}

DANG Ruihua ^{[1
]}

机构：

[1] College of Animal Science and Technology,Northwest A&F University

[2] Institute of Hexi Ecology and Oasis Agriculture,Hexi University

来源：

Journal of Integrative Agriculture | 2017年 / 16卷 / 03期

关键词：

Chinese donkey; myostatin gene; SNP; genetic diversity;

D O I：

暂无

中图分类号：

S822 [驴、骡];

学科分类号：

0905 ;

摘要：

Myostatin(MSTN)gene negatively controls skeletal muscle development and growth,variations of which play an important role in the regulation of skeletal muscle growth in mammals.However,study on genetic polymorphism of MSTN gene in donkey is limited.In this study,we screened the single nucleotide polymorphsims(SNPs)of MSTN gene in 13 Chinese donkey breeds.Four novel SNPs(g.229T>C,g.872A>G,g.2014G>A,and g.2395C>G)were detected and genotyped by sequencing and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)methods.Six haplotypes(H1–H6)were analyzed,which indicated abundant haplotype diversities in Chinese donkeys.The haplotype H1 was the most dominant and ancient in all breeds.Xinjiang donkey displayed the highest haplotype diversity.The Neighbour-Joining(NJ)tree of MSTN gene among different species was constructed.The clustering result of nine species was consistent with the fact of species differentiation.Our results will provide a reliable theoretical basis for the preservation,exploration and utilization of Chinese donkey genetic resources.

引用

页码：656 / 663

页数：8

共 13 条

[11]

Identification of the myostatin locus (MSTN) as having a major effect on optimum racing distance in the Thoroughbred horse in the USA[J] . M. M.Binns,D. A.Boehler,D. H.Lambert. Animal Genetics . 2010

[12]

Analysis of Horse Myostatin Gene and Identification of Single Nucleotide Polymorphisms in Breeds of Different Morphological Types[J] . Stefania Dall’Olio,Luca Fontanesi,Leonardo Nanni Costa,Marco Tassinari,Laura Minieri,Adalberto Falaschini,Peter Dovc. Journal of Biomedicine and Biotechnology . 2010

[13]

MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences[J] . Sudhir Kumar,Masatoshi Nei,Joel Dudley,Koichiro Tamura. Briefings in Bioinformatics . 2008 (4)

← 1 2 →