The genetic diversity analysis in the donkey myostatin gene

被引：0

作者：

LIU Donghua ^{[1
,2
]}

HAN Haoyuan ^{[1
]}

ZHANG Xin ^{[1
]}

SUN Ting ^{[1
]}

LAN Xianyong ^{[1
]}

CHEN Hong ^{[1
]}

LEI Chuzhao ^{[1
]}

DANG Ruihua ^{[1
]}

机构：

[1] College of Animal Science and Technology,Northwest A&F University

[2] Institute of Hexi Ecology and Oasis Agriculture,Hexi University

来源：

Journal of Integrative Agriculture | 2017年 / 16卷 / 03期

关键词：

Chinese donkey; myostatin gene; SNP; genetic diversity;

D O I：

暂无

中图分类号：

S822 [驴、骡];

学科分类号：

0905 ;

摘要：

Myostatin(MSTN)gene negatively controls skeletal muscle development and growth,variations of which play an important role in the regulation of skeletal muscle growth in mammals.However,study on genetic polymorphism of MSTN gene in donkey is limited.In this study,we screened the single nucleotide polymorphsims(SNPs)of MSTN gene in 13 Chinese donkey breeds.Four novel SNPs(g.229T>C,g.872A>G,g.2014G>A,and g.2395C>G)were detected and genotyped by sequencing and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)methods.Six haplotypes(H1–H6)were analyzed,which indicated abundant haplotype diversities in Chinese donkeys.The haplotype H1 was the most dominant and ancient in all breeds.Xinjiang donkey displayed the highest haplotype diversity.The Neighbour-Joining(NJ)tree of MSTN gene among different species was constructed.The clustering result of nine species was consistent with the fact of species differentiation.Our results will provide a reliable theoretical basis for the preservation,exploration and utilization of Chinese donkey genetic resources.

引用

页码：656 / 663

页数：8

共 13 条

[1]

Deletion of TTTTA in 5'UTR of goat MSTN gene and its distribution in different population groups and genetic effect on bodyweight at different ages[J]. Xianglong LI, Zhengzhu LIU, Rongyan ZHOU, Guiru ZHENG, Yuanfang GONG, Lanhui LI College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China Department of Animal Science, Hebei Normal University of Science & Technology, Changli 066600, China. Frontiers of Agriculture in China. 2008 (01)

[2] 鸡Myostatin基因单核苷酸多态性的群体遗传学分析 [J].

顾志良 ;

张海峰 ;

朱大海 ;

李辉 .

遗传学报, 2002, (07) :599-606

[3] 猪MSTN基因多态性及其SNPs的研究 [J].

李绍华 ;

熊远著 ;

郑嵘 ;

李爱云 ;

邓昌彦 ;

蒋思文 ;

雷明刚 ;

文雅芹 ;

曹国春 .

遗传学报, 2002, (04) :326-331

[4] Haplotype diversity in the equine myostatin gene with focus on variants associated with race distance propensity and muscle fiber type proportions [J].

Petersen, Jessica L. ;

Valberg, Stephanie J. ;

Mickelson, James R. ;

McCue, Molly E. .

ANIMAL GENETICS, 2014, 45 (06) :827-835

[5]

Association of myostatin ( MSTN ) gene polymorphisms with morphological traits in the Italian Heavy Draft Horse breed[J] . S. Dall’Olio,Y. Wang,C. Sartori,L. Fontanesi,R. Mantovani. Livestock Science . 2013

[6] Single nucleotide polymorphisms in the upstream regulatory region alter the expression of myostatin [J].

Hu, Wei ;

Chen, Songyu ;

Zhang, Ran ;

Lin, Yushuang .

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2013, 49 (06) :417-423

[7] Horses for courses [J].

Sartorius, N. .

EPIDEMIOLOGY AND PSYCHIATRIC SCIENCES, 2012, 21 (03) :265-266

[8] Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis) [J].

Liu, Lusha ;

Yu, Xiaomu ;

Tong, Jingou .

MOLECULAR BIOLOGY REPORTS, 2012, 39 (09) :9211-9221

[9] A cohort study of racing performance in Japanese Thoroughbred racehorses using genome information on ECA18 [J].

Tozaki, T. ;

Hill, E. W. ;

Hirota, K. ;

Kakoi, H. ;

Gawahara, H. ;

Miyake, T. ;

Sugita, S. ;

Hasegawa, T. ;

Ishida, N. ;

Nakano, Y. ;

Kurosawa, M. .

ANIMAL GENETICS, 2012, 43 (01) :42-52

[10]

A genome‐wide association study for racing performances in Thoroughbreds clarifies a candidate region near the MSTN gene[J] . T.Tozaki,T.Miyake,H.Kakoi,H.Gawahara,S.Sugita,T.Hasegawa,N.Ishida,K.Hirota,Y.Nakano. Animal Genetics . 2010

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