MiR-96-5p inhibition induces cell apoptosis in gastric adenocarcinoma

BACKGROUND Gastric adenocarcinoma(GAC) mortality rates have remained relatively changed over the past 30 years, and it continues to be one of the leading causes of cancerrelated death.AIM To search for novel mi RNAs related to GAC prognosis and further investigate the effect of mi R-96-5 p on MGC-803 cells.METHODS The mi RNA expression profile data of GAC based on The Cancer Genome Atlas were obtained and used to screen differently expressed mi RNAs(DEMs) and DEMs related to GAC prognosis. Then, the expression of DEMs related to GAC prognosis was identified in GAC tumor samples and adjacent normal samples by q RT-PCR. The target gene, ZDHHC5, of mi R-96-5 p was predicted using Target Scan, mi RTar Base, and mi RDB databases and confirmed by luciferase reporter assay. Furthermore, MGC-803 cells were transfected with inhibitor NC,mi R-96-5 p inhibitor, si-ZDHHC5, or mi R-96-5 p inhibitor + si-ZDHHC5, and then cell apoptosis was detected by flow cytometry. The expression of ZDHHC5, Bcl-2, and COX-2 was detected using western blotting.RESULTS A total of 299 DEMs and 35 DEMs related to GAC prognosis were screened based on The Cancer Genome Atlas. Then compared with adjacent normal samples, the levels of mi R-96-5 p, mi R-222-5 p, and mi R-652-5 p were remarkably increased,while mi R-125-5 p, mi R-145-3 p, and mi R-379-3 p levels were reduced in GAC tumor samples(P < 0.01), which were consistent with bioinformatics analysis.Furthermore, ZDHHC5 was defined as a direct target gene of mi R-96-5 p. mi R-96-5 p inhibition increased the number of apoptotic cells as well as promoted the expression of ZDHHC5, Bcl-2, and COX-2 in MGC-803 cells(P < 0.01). After ZDHHC5 inhibition, the number of apoptotic cells and the expression of ZDHHC5, Bcl-2, and COX-2 were reduced. The addition of an mi R-96-5 p inhibitor partly reversed these effects(P < 0.01).CONCLUSION Our findings identified six mi RNAs related to GAC prognosis and suggested that downregulated mi R-96-5 p might induce cell apoptosis via upregulating ZDHHC5 expression in MGC-803 cells.