Molecular Cloning and Prokaryotic Expression of Non-Structural Protein NS1 Gene of Porcine Parvovirus

被引：0
作者：
WU Dan TONG Guangzhi QIU Huaji XUE Qiang ZHOU Yanjun and LI Jingpeng National Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Harbin PRChinaDepartment of Biotechnology Northeast Agricultural University Harbin PRChina [1 ,2 ,1 ,1 ,1 ,1 ,2 ,1 ,150001 ,2 ,150030 ]
机构：
来源：
AgriculturalSciencesinChina | 2003年 / 10期
关键词：
Porcine parvovirus; NS1; Diagnosis;
D O I：
暂无
中图分类号：
S852.65 [家畜病毒学];
学科分类号：
090601 ;
摘要：
Porcine parvovirus (PPV) is one of the major agents causing swine reproductive failure. NS1 protein is a non-structural protein of PPV and can be used as a reagent for differentiation of vaccinated animals and infected ones. In present study, a recombinant plasmid pET28a/NS1 was constructed by cloning the coding sequence for NS1 of PPV into pET28a, a bacterial expression vector. The NS1 protein was expressed in E. coli BL21(DE3) after induced by IPTG and the recombinant fusion protein was purified with affinity chromatography. Expression amount of NS1 protein was improved by optimizing the inducing parameters. The recombinant NS1 protein is reactive to PPV positive sera in Western blot and ELISA test and therefore can be applicable in differential diagnosis of PPV infections.
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页码：117 / 120
页数：4
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