Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection

被引：0

作者：

Jiadai Li ^{[1
]}

Lingling Han ^{[1
]}

Yao Hao ^{[1
]}

Yuncong Yuan ^{[1
]}

Mingzhen Wang ^{[1
]}

Xiu Xin ^{[1
]}

Hailong Wang ^{[1
]}

Fang Yu ^{[2
]}

Congyi Zheng ^{[1
,3
]}

Chao Shen ^{[1
,3
]}

机构：

[1] State Key Laboratory of Virology, College of Life Sciences,Wuhan University

[2] Department of Pathology, Zhongnan Hospital, Wuhan University

[3] China Center for Type Culture Collection, Wuhan University

来源：

Virologica Sinica | 2020年 / 01期

基金：

中央高校基本科研业务费专项资金资助;

关键词：

Foot-and-mouth disease virus(FMDV); Persistent infection; RNA-Seq; Differentially expressed gene(DEG);

D O I：

暂无

中图分类号：

S852.65 [家畜病毒学];

学科分类号：

090601 ;

摘要：

Foot-and-mouth disease virus(FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome-and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1(Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication.

引用

页码：52 / 63

页数：12

共 10 条

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