Anti-aging effect of aqueous extract from Astragalus membranaceus on Drosophila melanogaster

被引：0

作者：

QIAO Yu-qi ^{[1
]}

ZHANG Jian-qin ^{[1
]}

QIN Xue-mei ^{[1
]}

机构：

[1] Modern Research Center For Traditional Chinese Medicine, Shanxi University

来源：

中国药理学与毒理学杂志 | 2019年 / 10期

基金：

新加坡国家研究基金会;

关键词：

Astragalus membranaceus; Drosophila melanogaster; life span; anti-aging; oxidative stress;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

OBJECTIVE To study the anti-aging effect of aqueous extract from Astragalus membranaceus on Drosophila melanogaster. METHODS Drosophila（male and female） were collected within 3 d in adult stage.Drosophila were randomly divided into control group and aqueous extract from Astragalus groups(0.625, 1.25,2.5, 5 and 10 g·L;). The pharmacodynamics experiment of aqueous extract from Astragalus was investigated by using lifespan experiment, crawling assay, hydrogen peroxide stress assay, paraquat stress assay. To evaluate the antioxidant effect of aqueous extract from Astragalus,the enzyme activity of superoxide dismutase（SOD）, catalase（CAT） were conducted. RESULTS The results of lifespan showed that 1.25 g·L;of aqueous extract from Astragalus could significantly prolong the life span of both male and female Drosophila（P<0.05）. The 0.625 g·L;of aqueous extract from Astragalus could significantly extend the life span of female Drosophila（P<0.01）. The1.25 g·L;of aqueous extract from Astragalus could significantly improve the crawling ability of male Drosophila at 25 d age（P<0.05）. The 1.25 g·L;of aqueous extract from Astragalus significantly extended the life span of male Drosophila after hydrogen peroxide exposure（P<0.01）. The 0.625 g·L;of aqueous extract from Astragalus could significantly prolong the life span of female Drosophila after hydrogen peroxide exposure（P<0.05）. The 1.25 g·L;of aqueous extract from Astragalus could significantly prolong the life span of male and female Drosophila after paraquat exposure（P<0.01）. The 1.25 g·L;of aqueous extract from Astragalus significantly increased SOD and CAT activity in fruit flies at 25 d age（P<0.01）. CONCLUSION The aqueous extract from Astragalus prolongs the life span of Drosophila by improving the activity of SOD,CAT and alleviating the damage of free radicals caused by aging.

引用

页码：849 / 849

页数：1