Application Research of Complex Enzyme on the Extraction of Effective Components from HERBA PHYLLANTHI URINARIAE

[Objective] To optimize the complex enzyme-assisted extraction process of effective components from HERBA PHYLLANTHI URINARIAE,so as to provide references for its high-efficient utilization.[Method] The flavonoids were extracted from HERBA PHYLLANTHI URINARIAE by using water as the extracting solvent.Using the yield of total flavonoids as index,the extraction conditions were optimized by the orthogonal test with 4 levels and 5 factors,namely,material-liquid ratio,extraction temperature,extraction time,cellulase concentration and pectase concentration.[Result] The yield of total flavonoids in extracts was the highest when fine powder was refluxed for 2 h at 40 ℃ with 25 times of water as extracting solvent in the presence of 1.4 g/L cellulase and 2.0 g/L pectase.Under the optimized conditions,the yield of total flavonoids was 1.589%.The influence of five inspected factors on the yield of total flavonoids decreased in the order of extraction temperature > pectase concentration > material-liquid ratio > extraction time > cellulase concentration.[Conclusion] Complex enzyme-assisted extraction method was rapid,efficient and environmental-friendly with low carbon consumption and high extraction yield.