Quantifying anti-HBV effect of targeted ribonuclease by real-time fluorescent PCR

被引:0
|
作者
Jun Liu Ying-Hui Li Jin Ding Wei-Dong Gong Cai-Fang Xue Ya Zhao Yu-Xiao Huang Department of Etiology
机构
基金
中国国家自然科学基金;
关键词
HBV; Quantifying anti-HBV effect of targeted ribonuclease by real-time fluorescent PCR; real; time;
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暂无
中图分类号
R450 [];
学科分类号
100215 ;
摘要
AIM:To quantify the inhibition of HBV replication by targetedribonuclease by using real-time fluorescent PCR.METHODS:Targeted ribonuclease was introduced into 2.2.15cells by liposome-mediated transfection or HIV-TAT mediatedprotein transduction.Forty-eight hours after the transfectionand 24 h after the transduction,supernatants of 2.2.15 cellswere collected and HBV DNA in the supernatants wasquantified by real-time fluorescent PCR with a commercial kit.RESULTS:HBV DNA concentrations in the supernatants of2.2.15 cells transfected or transducted with targetedribonuclease were 4.9±2.4×10~8 copies/L and 8.3±4.0×10~8copies/L,respectively.Compared with controls,transfectionor transduction of targeted ribonuclease reduced HBV DNAconcentration in the supematants of 2.2.15 cells by 90.4%and 90.1%,respectively(P<0.05).CONCLUSION:Targeted ribonuclease can inhibit HBVreplication in 2.2.15 cells.
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页码:2883 / 2885
页数:3
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