Establishment of Specific Chromatogram of Spica Prunellae Formula Granules Based on Standard Decoction

被引：0

作者：

Xinwei NONG ^{[1
]}

Mei YANG ^{[1
]}

Xiongmei HUANG ^{[1
]}

Huilian DENG ^{[1
]}

Chan WANG ^{[1
]}

Jian ZHOU ^{[1
]}

机构：

[1] Pura Pharm (Nanning) Pharmaceutical Co.,Ltd.

来源：

Medicinal Plant | 2019年 / 06期

关键词：

Spica Prunellae; Standard decoction; Formula granule; HPLC; Fingerprint chromatogram;

D O I：

10.19600/j.cnki.issn2152-3924.2019.06.019

中图分类号：

R286.0 [药品鉴定];

学科分类号：

1008 ;

摘要：

[Objectives] This study aimed to establish HPLC chromatograms of decoction pieces, standard decoction and formula granules of Spica Prunellae. [Methods] The chromatographic conditions were as follows: column, SHISEIDO CAPCELL PAK C;MGII column（4.6 mm×250 mm, 5 μm）; mobile phase, acetonitrile-0.1% phosphoric acid; gradient elution; detection wavelength, 280 nm; flow rate, 1.0 mL/min; and column temperature, 30 ℃. The correlation between the decoction pieces, standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms. [Results] The fingerprint chromatograms of decoction pieces, standard decoction and formula granules of Spica Prunellae showed five common peaks, with good correlation. Among the five common peaks, four of them were tanshinol, protocatechuic acid, caffeic acid and rosmarinic acid. [Conclusions] The main chemical constituents of decoction pieces, standard decoction and formula granules of Spica Prunellae are basically the same. The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.

引用

页码：68 / 71

页数：4