Determination of Hypericin Content in Hypericum perforatum L. by HPLC

[Objective] To determine the content of hypericin in Hypericum perforalurn L.by HPLC,so as to favor the development of Hypericum perforalurn L..[Method] HPLC conditions were as follows:column of SHIMADZU VP-ODS C18 column,mobile phase of methanol-ethyl acetate-phosphate buffer(V:V:V=60:20:20,pH=2.0),flow rate of 1.0 ml/min,column temperature of 25 ℃,detection wavelength of 590 nm and sampling amount of 10 μl.[Result] In the range of 2.56-19.20 μg/ml,the linear relationship between content of hypericin and its peak area was good(r=0.999 9).The content of hypericin in Hypericum perforalurn L.was determined to be 0.79 mg/g and the yield was 0.079%.[Conclusion] The method established in this study with good precision,reproducibility and stability is favorable for the determination of hypericin in Hypericum perforalurn L.,which provides evidence for the development of Hypericum perforalurn L..