Production and Characterization of Polyclonal Antibody for the N-Methylcarbamate Insecticide Metolcarb

被引：0
作者：
ZHANG Qi1
机构：
来源：
AgriculturalSciencesinChina | 2007年 / 02期
关键词：
metolcarb; antigen; polyclonal antibody; antiserum characterization; ELISA;
D O I：
暂无
中图分类号：
S482.3 [杀虫剂];
学科分类号：
090403 ;
摘要：
The hapten, 3-{[1-(3-(methyl)phenyloxy)-carbonyl]amino}propanoic acid (HOM), mimicking the analyte metolcarb, was synthesized and verified by mass spectrometry (MS) and 1H-nuclear magnetic resonance spectrometry (1H-NMR). Then, HOM was conjugated with the carrier proteins bovine serum albumin (BSA) and ovalbumin (OVA) with stoichiometric amounts of N-hydroxysuccinimide/dicyclohexylcarbodimide (NHS/DCC) using the activated ester method. Polyclonal antibodies were raised against the conjugate of HOM-BSA in rabbits. Antiserum titres were determined by noncompetitive indirect ELISA procedures and the titer of pAb01 reached 1.28 × 106. The cross-reactivities of the structurally related N- methylcarbamate insecticides were 0.0% except for dimethacarb. These results indicate that the antibody pAb01 with strong affinity and high specificity can be used to develop a sensitive and rapid detection protocol for metolcarb residue.
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页码：175 / 179
页数：5
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