Recovery of DNA from Agarose Gel with Home-made Silica Milk

An usefulness of silica milk made wi th waste ultraviolet light tube for recovery of DNA fragment from agarose gel was r epresented. The glass milk is a water suspension of 50% fine silica powder prepa red by grinding the crushed waste ultraviolet light tube with a porcelain mortar .It was showed that one microliter of the glass milk could bind more than 1 μg of DNA fragment,and DNA fragment in length from 125 bp to 23 kb could be efficie ntly recovered from agarose gel. The bound DNA could be eluted from the particle of SiO 2 in the glass milk with a yield of about 60%\|80%.The eluted DNA could be used in all manipulations in molecular cloning.