A panel of monoclonal antibodies against the prion protein proves that there is no prion protein in human pancreatic ductal epithelial cells

被引：0

作者：

Liheng Yang ^{[1
,2
]}

Yan Zhang ^{[3
]}

Lipeng Hu ^{[1
]}

Ying Zhu ^{[2
]}

Man-Sun Sy ^{[4
]}

Chaoyang Li ^{[1
]}

机构：

[1] State Key Laboratory of Virology and Department of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences

[2] Department of Virology, School of Life Sciences, Wuhan University

[3] Department of Molecular Endocrinology, Institute of Hydrobiology, Chinese Academy of Sciences

[4] Department of Pathology, Case Western Reserve University

来源：

Virologica Sinica | 2014年 / 04期

关键词：

prion protein; monoclonal antibody; human pancreatic ductal epithelial cells;

D O I：

暂无

中图分类号：

R373.9 [其他病毒];

学科分类号：

100103 ; 100705 ;

摘要：

Prion diseases are a group of neurodegenerative diseases that are fatal. The study of these unique diseases in China is hampered by a lack of resources. Amongst the most important resources for biological study are monoclonal antibodies. Here, we characterize a panel of monoclonal antibodies specific for cellular prion protein by enzyme-linked immunosorbent assay(ELISA), immunofluorescent staining, flow cytometry, and western blotting. We identify several antibodies that can be used for specific applications and we demonstrate that there is no prion protein expression in human pancreatic ductal epithelial cells(HPDC).

引用

页码：228 / 236

页数：9

共 2 条

[1]

Clinical and Familial Characteristics of Ten Chinese Patients with Fatal Family Insomnia[J]. SHI Qi1,CHEN Cao1,GAO Chen1,TIAN Chan1,ZHOU Wei1,ZHANG BaoYun1,HAN Jun1,and DONG Xiao Ping1,2,# 1.State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;2.Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Science

[2]

Immunological detection of conformational neoepitopes associated with the serpin activity of plasminogen activator inhibitor type-2 .2 Saunders D N,Buttigieg K M,Gould A,McPhun V,Baker M S. The Journal of biological chemistry . 1998

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