miR-136通过抑制PPP2R2A表达调控胃癌细胞SGC7901凋亡

被引：2

作者：

邢亚楠

邓鹏

徐惠绵

机构：

[1] 中国医科大学附属第一医院肿瘤外科

来源：

临床与病理杂志 | 2015年 / 35卷 / 12期

关键词：

SGC7901细胞; miR-136; PPP2R2A; 增殖; 凋亡;

D O I：

暂无

中图分类号：

R735.2 [胃肿瘤];

学科分类号：

摘要：

目的:检测miR-136对胃癌细胞SGC7901增殖和凋亡的影响及其机制。方法:分别利用MTT实验和Annex in V-PI实验检测转染miR-136 inhibitor的胃癌细胞SGC7901增殖和凋亡。利用western blot和双荧光素酶报告基因技术检测miR-136的靶基因。结果:抑制miR-136表达可以明显抑制SGC7901细胞增殖和诱导其凋亡。miR-136能够与PPP2R2A mRNA 3'-UTR区结合。结论:抑制miR-136表达能够促进PPP2R2A蛋白表达,进而引起胃癌细胞凋亡。

引用

页码：2108 / 2111

页数：4

共 5 条

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