Changes in Protein Content and Chitinase and β-1,3-glucanase Activities of Rice with Blast Resistance Induced by Ag-antibiotic 702

[Objective] The paper was to explore the effect of rice blast resistance induced by Ag-antibiotics 702 on protein content and chitinase and β-1,3-glucanase activities in rice. [Method]At the fourth leaf stage of Luliangyou 996,15 μg/m L Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. All treatments were inoculated with spore fluid of Magnaporthe grisea at 48 h post spraying,and the rice inoculated with only distilled water was used as blank control. The enzymes activities( endochitinase,exochitinase and β-1,3-glucanase) and total protein content in rice leaves were determined every 24 h within 168 h post spraying. [Result] Compared with the blank control,the rice inoculated with spore fluid of M. grisea could significantly increase the total protein content and the activities of β-1,3-glucanase and chitinase. The induction effect of Ag-antibiotics 702 exceeded that of Validamycin treatment. And the changes in activities of β-1,3-glucanase and chitinase had obvious synchronicity. [Conclusion] Ag-antibiotic 702 can significantly improve the total protein content and the activities of β-1,3-glucanase and chitinase,thus enhancing the resistance to rice blast.