人类微小RNA-92a-2-5p调控HEATR1基因对胃癌细胞的生物学功能

被引：2

作者：

周先飞 ^{[1
]}

谭骞 ^{[1
]}

林锡冬 ^{[1
]}

赵飞龙 ^{[1
]}

杨年钊 ^{[2
]}

赵军 ^{[2
]}

机构：

[1] 皖南医学院研究生院, 芜湖

[2] 皖南医学院第一附属医院弋矶山医院胃肠外科

来源：

中华实验外科杂志 | 2023年 / 40卷 / 02期

基金：

安徽省自然科学基金;

关键词：

胃癌; 微小RNA; 标志物;

D O I：

暂无

中图分类号：

R735.2 [胃肿瘤];

学科分类号：

摘要：

目的阐明人类微小RNA(hsa-miR)-92a-2-5p可通过抑制HEATR1基因对胃癌细胞生物学作用。方法采用在线生物信息学工具(Target Scan)以及文献调研进行统计筛选, 选择结合较好的5种微小RNA(miRNA):hsa-miR-92a-2-5p、hsa-miR-20a-2-3p、hsa-miR-183-5p、hsa-miR-506-3p和hsa-miR-556, 通过蛋白质印迹法(Western blot)对5种miRNAs在两种胃癌细胞株(AGS、MGC803)检测目的基因的蛋白的表达变化。使用细胞计数试剂盒(CCK-8)实验、PI-FACS细胞周期实验、克隆形成实验检测细胞增殖情况、流式细胞仪检测细胞凋亡、迁移及侵袭实验检测细胞的迁移、侵袭能力, 组间比较采用t检验。结果 hsa-miR-92a-2-5p被证明与HEATR1存在较好的调控关系。过表达hsa-miR-92a-2-5p后, AGS、MGC803细胞中转染组克隆形成能力低于阴性对照组(83.000±2.646比150.667±7.638, t=14.500, P<0.01;264.667±9.713比288.333±6.658, t=3.481, P<0.05)。在培养96 h及120 h后, CCK-8实验显示转染组增殖能力低于阴性对照组, AGS细胞(0.380±0.032比0.612±0.019, t=10.621, P<0.01;0.912±0.093比1.590±0.008, t=12.585, P<0.01), MGC803细胞(0.588±0.042比0.967±0.025, t=13.691, P<0.01;1.052±0.028比1.559±0.013, t=28.550, P<0.01)。细胞凋亡实验显示, AGS、MGC803细胞细胞中转染组凋亡率高于阴性对照组(12.667±0.950比5.727±0.482, t=11.279, P<0.01;16.133±2.937比5.560±1.361, t=5.658, P<0.01)。AGS和MGC803细胞侵袭、转移能力转染组均低于阴性对照组, AGS细胞中(360.000±7.937比550.667±10.017, t=25.840, P<0.01;265.333±12.503比455.667±9.292, t=21.163, P<0.01), MGC803细胞中(139.667±6.110比241.000±5.568, t=21.232, P<0.01;61.333±9.292比218.333±4.619, t=26.207, P<0.01)。结论 hsa-miR-92a-2-5p可以敲减HEATR1基因的表达, 抑制胃癌细胞增殖、侵袭、转移, 并促进胃癌细胞的凋亡。

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