转cry1Ab/cry1Ac基因玉米Cry1Ab/Cry1Ac融合蛋白表达及对亚洲玉米螟的室内杀虫效果

被引：4

作者：

刘晓贝 ^{[1
,2
]}

白树雄 ^{[2
]}

王振营 ^{[2
]}

王月琴 ^{[2
]}

王勤英 ^{[1
]}

何康来 ^{[2
]}

机构：

[1] 河北农业大学植物保护学院

[2] 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室

来源：

昆虫学报 | 2020年 / 63卷 / 10期

关键词：

亚洲玉米螟; 转cry1Ab/cry1Ac基因玉米; 杀虫效果; 蛋白表达量; 室内生测;

D O I：

10.16380/j.kcxb.2020.10.005

中图分类号：

S435.132 [玉米虫害];

学科分类号：

摘要：

【目的】室内抗螟性评价是转Bt基因抗虫玉米研发和安全性评价的重要环节。【方法】采用酶联免疫吸附测定法(ELISA)测定了转cry1Ab/cry1Ac基因玉米ZZM030心叶中Cry1Ab/Cry1Ac融合杀虫蛋白的表达量;采用室内生测法测定了分别取食转基因玉米ZZM030和非转基因玉米X249心叶后亚洲玉米螟Ostrinia furnacalis敏感品系ACB-BtS、Cry1Ab抗性品系ACB-AbR和Cry1Ac抗性品系ACB-AcR初孵幼虫的存活率。【结果】转基因抗虫玉米ZZM030 4叶期和8叶期心叶中Cry1Ab/Cry1Ac融合杀虫蛋白的表达量分别是10.62和2.94μg/g FW。敏感品系亚洲玉米螟初孵幼虫取食转基因玉米ZZM030心叶2 d的存活率仅为23.6%,4 d后存活率为0,而取食非转基因对照玉米X249心叶4 d的存活率高达93.1%。Cry1Ab抗性品系和Cry1Ac抗性品系初孵幼虫取食转基因玉米ZZM030心叶6 d后的存活率分别为11.1%和12.5%,而取食非转基因玉米X249心叶6 d后的存活率分别为81.9%和77.8%。【结论】转cry1Ab/cry1Ac基因玉米ZZM030心叶中高表达的Cry1Ab/Cry1Ac融合蛋白对亚洲玉米螟初孵幼虫具有极高的杀虫效果。

引用

页码：1201 / 1206

页数：6

共 28 条

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