Application of PCR-DGGE in Research of Bacterial Diversity in Drinking Water

被引：0
作者：
QING WU2
机构：
来源：
BiomedicalandEnvironmentalSciences | 2006年 / 05期
基金：
美国国家科学基金会;
关键词：
Drinking water; Denaturing gradient gel electrophoresis (DGGE); 16S ribosome RNA; Microbial diversity;
D O I：
暂无
中图分类号：
R123.1 [水的卫生标准和水的卫生监测];
学科分类号：
100402 ;
摘要：
Objective To analyze the structure of bacteria in drinking water by molecular biological techniques. Methods DNA of bacteria in drinking water was directly extracted without culture. 16S ribosomal DNA fragments, including V-6, -7, and -8 regions, were amplified with universal primers (EUBf933GC and EUBr1387) and analyzed by DGGE. Results DGGE indicated that amplification products could be separated. The results showed that DGGE could be used in the separation of different microbial 16SrRNA genes extracted from drinking water. Though there were special bacteria in different water samples, the predominant bacteria were essentially the same. Three sequences of the reclaimed specific bands were obtained, and phylogenetic tree of these bands was made. Conclusion Bacterial diversity in drinking water is identified by molecular biological techniques.
引用
收藏
页码：371 / 374
页数：4
相关论文
共 9 条
  • [1] 16S ribosomal DNA-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by PCR and denaturing gradient gel electrophoresis. Mako Kawai,Eiichi Matsutera. Appl Environ Microbiol . 2002
  • [2] Detection methods in water and waste water.. . 2002
  • [3] Phylogenetic analysis of pathogenic anaerobe clostridium perfringens using the 16SrRNA nucleotide sequence. Canard B,,Garnier T,Lafay B, et al. Int J Syst Bacteriol . 1992
  • [4] Application of 16SrRNA in the detection of bacteria in mouth. Zhang R,Li Y. Foreign medical sciences . 2000
  • [5] The ribosomal database project. Larsen N,Olsen G J,Maidak B L, et al. Nucleic Acids Res . 1993
  • [6] Application of 16S rRNA sequence analysis for the study of atmospheric bacteria. Zhou Y,Chen M L,Jiang L, et al. Letters in biotechnology . 2000
  • [7] Matching molecular diversity and ecophysiology of benthic cyanobacteria and diatoms in communities along a salinity gradient. Nubel U,,Garcia-Pichel F,Clavero E, et al. Environ Microbiol . 2000
  • [8] The application of 16SrRNA in molecular classification, identification and molecular examination in marine microbic system. Hong Y G,Sun M,Zhang Y B, et al. Marine fisheries research . 2002
  • [9] Monitoring impact of in situ biostimulation treatment on groundwater bacterial community by DGGE. Iwamoto, T,,K Tani,,K Nakamura, et al. FEMS Microbiol Ecol . 2000
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