Influence of liver nonparenchymal cell infusion combined with cyclosporin A on rejection of rat small bowel transplantation

AIM:To investigate the effect of liver nonparenchymal cellinfusion combined with cyclosporin A(CsA)on rejection ofheterostrain rat small bowel transplantation.METHODS:The liver nonparenchymal cell suspension wasprepared by density gradient centrifugation method withPercoll centrifugal solution.Heterotopic small boweltransplantation was performed.Then the rats were dividedinto four groups.Group one:homogenic transplantation(F344/N→F344/N),group two:allotransplantation(F344/N→Wistar),group three:allotransplantation(F344/N→Wistar)+CsA,with CsA 10 mg·kg-1·d-1after transplantation,groupfour:allotransplantation+CsA(F344/N→Wistar)+livernonparenchymal cell infusion+CsA(F344/N→Wistar),inwhich recipient Wistar rats had been injected with 2×10~8F344/N liver nonparenchymal cells 20 days beforetransplantation,and treated with CsA after transplantation.Finally,the survival time after small bowel transplantation,gross and histopathological examination,and IL-2 levels inserum were observed.RESULTS:The survival time alter small bowel transplantationwas 7.14±0.33 d,16.32±0.41 d and 31.41±0.74 d in group2,3,and 4,respectively.The survival time was significantlonger(P<0.01)in group 4.The gross and histopathologicalexamination showed that the rejection degree in group 4was lower than those in groups 2 and 3.Serum IL-2 level ingroup 4 was also lower than those in groups 2 and 3(P<0.01).CONCLUSION: Liver nonparenchymal cell infusion combined with CsA can prolong the survival time of rat small bowel transplantation, and the anti-rejection effect is good.