STAT3 mediates resistance of CD44~+CD24-/low breast cancer stem cells to tamoxifen in vitro

被引：0
作者：
Xiaoyan Wanga
bLaboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering
机构：
来源：
The Journal of Biomedical Research | 2012年 / 05期
关键词：
STAT3; breast cancer; cancer stem cells; tamoxifen drug resistance;
D O I：
暂无
中图分类号：
R737.9 [乳腺肿瘤];
学科分类号：
100214 ;
摘要：
We sought to determine whether STAT3 mediated tamoxifen resistance of breast cancer stem cells in vitro.The capacities for mammosphere formation and STAT3 expression of CD44+CD24-/low MCF-7 and MCF-7 were observed.The CD44+CD24-/low subpopulation ratio and its sensitivity to adriamycin were analyzed in MCF-7 and TAM resistant(TAM-R) cells.Cell cycle,apoptosis,STAT3 and phospho-STAT3 changes were observed af-ter treatment with tamoxifen.Small interference RNA-mediated knockdown of STAT3 in TAM-R cells was also performed.CD44+CD24-/low MCF-7 showed higher capacities for mammosphere formation and STAT3 expression than total MCF-7.The CD44+CD24-/low subpopulation was also upregulated in TAM-R cells with less sensitivity to adriamycin than MCF-7.Cell cycle changes,anti-apoptotic effects and STAT3 changes were also found.Mean-while,the knock-down of STAT3 in TAM-R resulted in an increase in sensitivity to tamoxifen.It is concluded that STAT3 plays an essential role in breast cancer stem cells,which correlated with tamoxifen resistance.
引用
收藏
页码：325 / 335
页数：11
相关论文
共 10 条
[1]   无血清悬浮法培养MCF-7细胞系并筛选该细胞系中肿瘤干细胞相关亚群的初步研究 [J].
许立生 ;
王水 ;
许健 ;
杜青 .
中华肿瘤防治杂志, 2007, (10) :736-740
[2]   Methylome analysis reveals Jak-STAT pathway deregulation in putative breast cancer stem cells [J].
Hernandez-Vargas, Hector ;
Ouzounova, Maria ;
Le Calvez-Kelm, Florence ;
Lambert, Marie-Pierre ;
McKay-Chopin, Sandrine ;
Tavtigian, Sean V. ;
Puisieux, Alain ;
Matar, Chantal ;
Herceg, Zdenko .
EPIGENETICS, 2011, 6 (04) :429-440
[3]   The potential role of estrogen receptors and the SRC family as targets for the treatment of breast cancer [J].
Spears, Melanie ;
Bartlettt, John .
EXPERT OPINION ON THERAPEUTIC TARGETS, 2009, 13 (06) :665-674
[4]   The role and regulation of the nuclear receptor co-activator AIB1 in breast cancer [J].
Lahusen, Tyler ;
Henke, Ralf T. ;
Kagan, Benjamin L. ;
Wellstein, Anton ;
Riegel, Anna T. .
BREAST CANCER RESEARCH AND TREATMENT, 2009, 116 (02) :225-237
[5]  
Ovarian steroids and the human breast: Regulation of stem cells and cell proliferation[J] . Robert B Clarke.Maturitas . 2006 (4)
[6]  
Effects of chemotherapy and hormonal therapy for early breast cancer on recurrence and 15-year survival: an overview of the randomised trials[J] . The Lancet . 2005 (9472)
[7]   EGFR dependent expression of STAT3 (but not STAT1) in breast cancer [J].
Berclaz, G ;
Altermatt, HJ ;
Rohrbach, V ;
Siragusa, A ;
Dreher, E ;
Smith, PD .
INTERNATIONAL JOURNAL OF ONCOLOGY, 2001, 19 (06) :1155-1160
[8]   Effective tamoxifen therapy of breast cancer involves both antiproliferative and pro-apoptotic changes [J].
Cameron, DA ;
Keen, JC ;
Dixon, JM ;
Bellamy, C ;
Hanby, A ;
Anderson, TJ ;
Miller, WR .
EUROPEAN JOURNAL OF CANCER, 2000, 36 (07) :845-851
[9]   Stat3 as an oncogene [J].
Bromberg, JF ;
Wrzeszczynska, MH ;
Devgan, G ;
Zhao, YX ;
Pestell, RG ;
Albanese, C ;
Darnell, JE .
CELL, 1999, 98 (03) :295-303
[10]   The expression of Ki-S1 and BCL-2 and the response to primary tamoxifen therapy in elderly patients with breast cancer [J].
Keen, JC ;
Dixon, JM ;
Miller, EP ;
Cameron, DA ;
Chetty, U ;
Hanby, A ;
Bellamy, C ;
Miller, WR .
BREAST CANCER RESEARCH AND TREATMENT, 1997, 44 (02) :123-133
1