Bay 11-7082增强Fas介导的HL-60细胞凋亡作用

被引:1
作者
王利
刘凌波
李蕾
邹萍
机构
[1] 华中科技大学同济医学院附属协和医院血液病研究所
关键词
Bay11-7082; Fas/FasL系统; HL-60; NF-κB;
D O I
暂无
中图分类号
R733.7 [白血病];
学科分类号
100214 ;
摘要
本研究观察NF-κB抑制剂Bay 11-7082对HL-60细胞Fas/FasL系统的作用及对Fas介导的HL-60细胞凋亡的影响。用RT-PCR和流式细胞术检测Bay 11-7082干预后HL-60细胞Fas、FasL和XIAP的mRNA和蛋白表达水平的变化;用ELISA方法检测Bay 11-7082干预前后sFasL水平的变化;流式细胞术检测Bay 11-7082干预前后CH-11(活性Fas抗体)诱导HL-60细胞凋亡的变化。结果表明:用Bay 11-7082干预后,HL-60细胞FasL和XIAP的mRNA和蛋白水平较对照组明显下降,差异具有显著性(p<0.05),Fas的mRNA、蛋白水平的变化和sFasL水平的变化无显著的差异(p>0.05);Bay 11-7082干预后HL-60细胞对CH-11诱导细胞凋亡敏感性显著增强。结论:Bay 11-7082可能通过下调FasL和XIAP水平增强HL-60细胞Fas介导的凋亡。
引用
收藏
页码:941 / 945
页数:5
相关论文
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