Objective To analyze the distribution and drug resistance of nontuberculous mycobacteria(NTM)in Beijing.Methods Using PCR-fluorescence probe method we identified 1 552 mycobacterial isolates in 2009 and 1 553mycobacterial isolates in 2013,which were stored by Beijing Research Institute for Tuberculosis Control.All identified NTM strains were confirmed by 16S rRNA gene