Phagocytosis of IgA Immune Complexes by Human U937 Cells

被引：0
|
作者：
郭彩云
崔薇
张伟
机构：
[1] Beijing 100005
[2] China
[3] Chinese Academy of Medical Sciences and Peking Union Medical College
[4] Institute of Basic Medical Sciences
[5] Peking Union Hospital
来源：
Journal of Microbiology and Immunology | 2003年 / 01期
关键词：
U937 cell FcR Immune complex IgA;
D O I：
暂无
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
In order to study FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells, antigen 8.9NIP/BSA was labeled with FITC and reacted with anti-NIP IgA or anti-NIP IgG antibody to form immune complexes (ICs). They were then incubated with phorbol 12-myristate 13-acetate (PMA) stimulated U937 cells.The phagocytosed ICs were quantified by flow cytometry. The results was that the expression of FcαRⅠ on U937 cells was higher than that of FcγRⅠ, FcγRⅡ and FcγRⅢ. After stimulation by PMA, expression of FcαRⅠ on U937 cells was markedly upregulated and the phagocytosis of IgA ICs was enhanced. FcαRⅠ mediated specific IgA phagocytosis was stronger than FcγRⅠ and FcγRⅡ mediated IgG phagocytosis. Complement receptors, CR1 and CR3, enhanced U937 cell phagocytosis of IgA ICs. It concludes that FcαRⅠ mediated strong phagocytosis of IgA ICs.
引用
收藏
页码：69 / 73
页数：5
相关论文
共 50 条
  • [21] Atromentin-Induced Apoptosis in Human Leukemia U937 Cells
    Kim, Jin Hee
    Lee, Choong Hwan
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 19 (09) : 946 - 950
  • [22] CATHEPSIN D IN DIFFERENTIATING U937 CELLS
    CELNIKER, A
    LUCAS, DO
    FEDERATION PROCEEDINGS, 1985, 44 (05) : 1702 - 1702
  • [23] The effects of minocyline on U937 cells.
    Amos, N
    Lawson, T
    Ethirmnnasingam, I
    Williams, BD
    ARTHRITIS AND RHEUMATISM, 1998, 41 (09): : S73 - S73
  • [24] Oxidized LDL immune complexes and oxidized LDL differentially affect the expression of genes involved with inflammation and survival in human U937 monocytic cells
    Hammad, Samar M.
    Twal, Waleed O.
    Barth, Jeremy L.
    Smith, Kent J.
    Saad, Antonio F.
    Virella, Gabriel
    Argraves, W. Scott
    Lopes-Virella, Maria F.
    ATHEROSCLEROSIS, 2009, 202 (02) : 394 - 404
  • [25] BINDING OF MODEL IMMUNE-COMPLEXES TO ERYTHROCYTE CR-1 FACILITATES IMMUNE-COMPLEX UPTAKE BY U937 CELLS
    EMLEN, W
    BURDICK, G
    CARL, V
    LACHMANN, PJ
    JOURNAL OF IMMUNOLOGY, 1989, 142 (12): : 4366 - 4371
  • [26] Phagocytotic Competence of Differentiated U937 Cells for Colloidal Drug Delivery Systems in Immune Cells
    Lessig, Jacqueline
    Neu, Bjoern
    Glander, Hans-Juergen
    Arnhold, Juergen
    Reibetanz, Uta
    INFLAMMATION, 2011, 34 (02) : 99 - 110
  • [27] Immune complexes induce TNF-α and BAFF production from U937 cells by HMGB1 and RAGE
    Gao, X. -J.
    Qu, Y. -Y.
    Liu, X. -W.
    Zhu, M.
    Ma, C. -Y.
    Jiao, Y. -L.
    Cui, B.
    Chen, Z. -J.
    Zhao, Y. -R.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2017, 21 (08) : 1810 - 1819
  • [28] Phagocytotic Competence of Differentiated U937 Cells for Colloidal Drug Delivery Systems in Immune Cells
    Jacqueline Leßig
    Björn Neu
    Hans-Jürgen Glander
    Jürgen Arnhold
    Uta Reibetanz
    Inflammation, 2011, 34 : 99 - 110
  • [29] High-density lipoprotein acts as an opsonin to enhance phagocytosis of group A streptococcus by U937 cells
    Liu, Ling
    Zhou, Lulei
    Li, Yuxin
    Bai, Wencheng
    Liu, Na
    Li, Wenlong
    Gao, Yumin
    Liu, Zhi
    Han, Runlin
    MICROBIOLOGY AND IMMUNOLOGY, 2015, 59 (07) : 419 - 425
  • [30] IMMUNE-COMPLEXES INDUCE IL-8 EXPRESSION FROM PROMONOCYTIC U937 CELLS AND HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS
    JARVIS, JN
    XU, CS
    ARTHRITIS AND RHEUMATISM, 1995, 38 (09): : 711 - 711
12345