Immunochemical identification and immunofluorescent localization of tropomyosin in germinated pollen of Lilium davidii

被引：0
|
作者：
任东涛
刘爱校
阎隆飞
机构：
[1] Beijing 100094
[2] China
[3] China Agrieultural University
[4] College of Biological Sciences
来源：
ProgressinNaturalScience | 1999年 / 03期
基金：
中国国家自然科学基金;
关键词：
lily pollen; tropomyosin; immunohlotting; immuno-fluorescence localization;
D O I：
暂无
中图分类号：
Q45 [内分泌生理学];
学科分类号：
071003 ;
摘要：
Tropomyosin, an actin-binding protein, can regulate the interaction between actin and myosm A kind of tropomyosin was identified and localized by anti-smooth muscle tropomyosm polyclonal antibody On im-munoblotting of the crude extract of lily pollen, antibody labels a polypeptide of approximately 64 kD. Immuno-fluorescence microscopy shows that the antibody yields numerous fluorescent spots throughout the whole length of pollen tube.
引用
收藏
页码：71 / 74
页数：4
相关论文
共 50 条
  • [31] Proteomic identification of differentially expressed proteins in mature and germinated maize pollen
    Zhu, Yunhao
    Zhao, Pengfei
    Wu, Xiaolin
    Wang, Wei
    Scali, Monica
    Cresti, Mauro
    ACTA PHYSIOLOGIAE PLANTARUM, 2011, 33 (04) : 1467 - 1474
  • [32] Proteomic identification of differentially expressed proteins in mature and germinated maize pollen
    Yunhao Zhu
    Pengfei Zhao
    Xiaolin Wu
    Wei Wang
    Monica Scali
    Mauro Cresti
    Acta Physiologiae Plantarum, 2011, 33 : 1467 - 1474
  • [33] IDENTIFICATION OF BRASSINOLIDE, CASTASTERONE, TYPHASTEROL, AND TEASTERONE FROM THE POLLEN OF LILIUM ELEGANS
    SUZUKI, H
    FUJIOKA, S
    YOKOTA, T
    MUROFUSHI, N
    SAKURAI, A
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (11) : 2075 - 2076
  • [34] SURVIVAL REACTIONS OF GERMINATED POLLEN FROM LILIUM LONGIFLORUM AFTER HEAT-STRESS WITHOUT HSP-FORMATION
    SCHRAUWEN, JAM
    VANHERPEN, MMA
    REIJNEN, WM
    WULLEMS, GJ
    ACTA BOTANICA NEERLANDICA, 1987, 36 (02): : 213 - 213
  • [35] Production and Identification of a Tetraploid Germplasm of Edible Lilium davidii var. unicolor Salisb via Chromosome Doubling
    Feng, Yayan
    Xu, Leifeng
    Yang, Panpan
    Xu, Hua
    Cao, Yuwei
    Tang, Yuchao
    Yuan, Suxia
    Ming, Jun
    HORTSCIENCE, 2017, 52 (07) : 946 - 951
  • [36] Isobaric Tags for Relative and Absolute Quantificationbased Comparative Proteomics Reveals the Features of Plasma Membrane-Associated Proteomes of Pollen Grains and Pollen Tubes from Lilium davidii
    Bing Han1
    Journal of Integrative Plant Biology, 2010, 52 (12) : 1043 - 1058
  • [37] The identification of a pollen-specific LEA-like protein in Lilium longiflorum
    Mogami, N
    Shiota, H
    Tanaka, I
    PLANT CELL AND ENVIRONMENT, 2002, 25 (05): : 653 - 663
  • [38] Isobaric Tags for Relative and Absolute Quantification-based Comparative Proteomics Reveals the Features of Plasma Membrane-Associated Proteomes of Pollen Grains and Pollen Tubes from Lilium davidii
    Han, Bing
    Chen, Sixue
    Dai, Shaojun
    Yang, Ning
    Wang, Tai
    JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2010, 52 (12) : 1043 - 1058
  • [39] Proteomics identification of differentially expressed proteins associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen
    Dai, Shaojun
    Chen, Taotao
    Chong, Kang
    Xue, Yongbiao
    Liu, Siqi
    Wang, Tai
    MOLECULAR & CELLULAR PROTEOMICS, 2007, 6 (02) : 207 - 230
  • [40] IMMUNOCHEMICAL AND IMMUNOCYTOCHEMICAL IDENTIFICATION OF A MYOSIN HEAVY-CHAIN POLYPEPTIDE IN NICOTIANA POLLEN TUBES
    TANG, XJ
    HEPLER, PK
    SCORDILIS, SP
    JOURNAL OF CELL SCIENCE, 1989, 92 : 569 - 574
12345