Astilbic acid induced COLO 205 cell apoptosis by regulating Bcl-2 and Bax expression and activating caspase-3

被引：0

作者：

Xiaoliang ZHENG Hongxiang SUN Xueli LIU Yunxiang CHEN Bochu QIANInstitute of Materia Medico Zhejiang Academy of Medical Sciences

College of Animal Sciences Zhejiang University

State Key Laboratory for New Drug Safety Evaluation Zhejiang Hangzhou China ^{[310013
]}

机构：

来源：

Acta Pharmacologica Sinica | 2004年 / 08期

关键词：

<Keyword>astilbic acid; COLO; 205; cells; apoptosis; cell cycle; bcl-2; genes; Bax protein; mitochondria; caspase;

D O I：

暂无

中图分类号：

R735.3 [肠肿瘤];

学科分类号：

100214 ;

摘要：

<正>AIM: To investigate the effect of astilbic acid (3β6β-dihydroxyolean-12-en-27-oic acidAA) on human colorectal carcinoma COLO 205 cell proliferation and apoptosis. METHODS: Proliferation of COLO 205 cells was measued by MTT assay. Content of DNA in COLO 205 cell was measued by modified diphenylamine assay. AA-induced morphological changes was observed with fluorescence microscope and transmission electron microscope . DNA fragmentation was visualized by agarose gel electrophoresis. Apoptosis rate and cell cycle distribution were determined by flow cytometric analysis. Expressions of Bcl-2 and Bax proteins were visioned by immunohistochemical analysis. The change of relative mitochondral transmembrane potential (MTP) in COLO 205 cell was analyzed with FCM after rhodamine 123 staining. RESULTS: The IC50 (96 h) of AA for inhibiting COLO 205 cell proliferation was 61.56±0.34 umol/L. AA induced a marked concentration- and time-dependent inhibition of COLO 205 cell proliferation and reduced the DNA content i

引用

页码：116 / 121

页数：6

共 19 条

[1]

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nicholson DW,Ali A,Thornberry NA,Vaillancourt JP,Ding CK,Gallant M,et al. Nature . 1995

[2]

Activation of CPP32-like proteases is not sufficient to trigger apoptosis, inhibition of apoptosis by agents that suppress activation of AP24, but not CPP32-like activity. Wright SC,Schellenberger U,Wang H,Kinder DH,TalhoukJW,Larrick JW. The Journal of Experimental Medicine . 1997

[3]

Glutathione antagonized cyclophosphamide and acrolein induced cyto-toxicity of PC3 cells and immunosuppressive actions in mice. Liu XL,Chen K,Ye YP,Peng XY,Qian BC. Acta Pharmacological Sinica . 1999

[4]

Bax suppresses tumorigenesis and stimulates apoptosis in vivo. Yin C,Knudson CM,Korsmeyer SJ,Van Dyke T. Nature . 1997

[5]

Effect of scopo letin on PC3 cell proliferation and apoptosis. Liu XL,Zhang L,Fu XL,Chen K,Qian BC. Acta Pharmacological Sinica . 2001

[6]

Effect of rhynchophylline on apoptosis induced by dopaminein NT2 cells. Shi JS,Haglid GK. Acta Pharmacological Sinica . 2002

[7]

Effects of droloxifene on apoptosis and Bax, Bcl-2 protein expression of luteal cells in pseudopregnant rats. Leng Y,Feng Y,Cao L,Gu ZP. Acta Pharmacological Sinica . 2001

[8]

Studies on anticancer activity of Astilbe chinensis. Chen PF,Lai PF,Zhang P,Chen LL,Li AP. China Journal of Chinese Materia Medica . 1996

[9]

Effect of recombinant human basic fibroblast growth factor on stomach ulcers in rats and mice. Wang JZ,Wu YJ,Rao CM,Gao MT,Li WG. Acta Pharmacological Sinica . 1999

[10]

A matter of life and cell death. Evan G,Littlewood T. Science . 1998

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