Identification of human genomic DNA from suppressed metastatic phenotype cells of mouse lung adenocarcinoma

被引:0
作者
Xueming Ge Yinglin Lu Shengfa Fu Kun Chen Wenhong Fan Shuang LiuDepartment of Molecular Pathologv Beijing Institute of Basic Medical Sciences Beijing [100850 ]
机构
关键词
lung; DNA; Identification of human genomic DNA from suppressed metastatic phenotype cells of mouse lung adenocarcinoma;
D O I
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中图分类号
R734.2 [肺肿瘤];
学科分类号
100214 ;
摘要
<正> Objectives:To isolate tumor metastaticsuppressing genes or relating human DNA sequencesand to study the molecular biological regulatingmechanism of tumor metastasis. Methods: HumanDNA fragments were amplified by Inter Alu PCRtechnique from normal human genomic DNAtransfected mouse tumor cell clones of which themetastatic phenotype had been suppressed. Thehuman origin of the amplified DNA was furtherconfirmed by PCR in situ hybridization. One DNA
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页码:294 / 295
页数:2
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